ARG70286

Human CD48 recombinant protein (ECD) (His-tagged, C-ter)

Human CD48 recombinant protein (ECD) (His-tagged, C-ter) for SDS-PAGE,Binding Activity and Human

Overview

Product Description HEK293 expressed, His-tagged (C-ter) Human CD48 recombinant protein (ECD).
Tested Reactivity Hu
Tested Application Binding, SDS-PAGE
Target Name CD48 (ECD)
Species Human
A.A. Sequnce Gln27 - Ser220 of Human CD48 (NP_001769.2) with 6X His tag at the C-terminus.
Expression System HEK293
Alternate Names Leukocyte antigen MEM-102; B-lymphocyte activation marker BLAST-1; CD48 antigen; BCM1 surface antigen; CD antigen CD48; TCT.1; BLAST1; BCM1; MEM-102; mCD48; Signaling lymphocytic activation molecule 2; BLAST; hCD48; SLAMF2; SLAM family member 2

Application Instructions

Application Note Binding activity test: Measured by its binding ability in a functional ELISA. Immobilized recombinant human CD48 at 5 ug/ml (100 µl/well) can bind recombinant human CD244 with a linear range of 0.2-1 ug/ml.

Properties

Form Powder
Purification Note 0.22 µm filter sterilized. Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4)
Reconstitution Reconstitute to a concentration of 0.1 - 0.5 mg/ml in sterile distilled water.
Storage Instruction Lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C for up to one month, at 2-8°C for up to one week. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 962 Human CD48

Swiss-port # P09326 Human CD48 antigen

Gene Symbol CD48
Gene Full Name CD48 molecule
Background This gene encodes a member of the CD2 subfamily of immunoglobulin-like receptors which includes SLAM (signaling lymphocyte activation molecules) proteins. The encoded protein is found on the surface of lymphocytes and other immune cells, dendritic cells and endothelial cells, and participates in activation and differentiation pathways in these cells. The encoded protein does not have a transmembrane domain, however, but is held at the cell surface by a GPI anchor via a C-terminal domain which maybe cleaved to yield a soluble form of the receptor. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]
Function Ligand for CD2. Might facilitate interaction between activated lymphocytes. Probably involved in regulating T-cell activation. [UniProt]
Cellular Localization Cell membrane; Lipid-anchor, GPI-anchor. [UniProt]
Calculated MW 28 kDa

Images (1) Click the Picture to Zoom In

  • ARG70286 Human CD48 recombinant protein (ECD) (His-tagged, C-ter) SDS-PAGE image

    SDS-PAGE analysis of ARG70286 Human CD48 recombinant protein (ECD) (His-tagged, C-ter).