ARG80128

Human MCP-1 / CCL2 ELISA Kit

Human MCP-1 / CCL2 ELISA Kit for ELISA and Human

Cancer kit; Cell Biology and Cellular Response kit; Immune System kit; Metabolism kit
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Component

Cat No Component Name Package Temp
ARG80128-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG80128-002 Standard (Lyophilized) 3 X 1 ng/vial 4°C
ARG80128-003 Standard diluent buffer 20 ml 4°C
ARG80128-004 Antibody conjugate concentrate 400 µl 4°C
ARG80128-005 Antibody diluent buffer 16 ml 4°C
ARG80128-006 HRP-Streptavidin concentrate 400 µl 4°C (Protect from light)
ARG80128-007 HRP-Streptavidin diluent buffer 16 ml 4°C
ARG80128-008 20X Wash buffer 50 ml 4°C
ARG80128-009 TMB substrate 12ml 4°C (Protect from light)
ARG80128-010 STOP solution 12ml 4°C
ARG80128-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG80128 Human MCP-1 / CCL2 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human MCP-1 / CCL2 in serum, plasma and cell culture supernatants.
Tested Reactivity Hu
Tested Application ELISA
Specificity No significant cross-reactivity or interference with Human CXCL-16, MCP-2, MIP-1 alpha, MIP-1 beta, RANTES, GRO/MGSA, GRO gamma, GRO beta, IL-8; Mouse MIP-1 alpha, MIP-1 beta.
Target Name CCL2 / MCP1
Conjugation HRP
Conjugation Note

Substrate: TMB and read at 450 nm

Sensitivity 8 pg/ml
Sample Type Serum, plasma and cell culture supernatants.
Standard Range 15.6 - 1000 pg/ml
Sample Volume 100 µl
Precision

CV: <10%

Alternate Names MCP1; Monocyte chemotactic and activating factor; MCAF; Monocyte chemotactic protein 1; Monocyte secretory protein JE; HSMCR30; Small-inducible cytokine A2; HC11; SMC-CF; GDCF-2; SCYA2; C-C motif chemokine 2; Monocyte chemoattractant protein 1; MCP-1

Application Instructions

Assay Time 4 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Symbol CCL2
Gene Full Name chemokine (C-C motif) ligand 2
Background This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of N-terminal cysteine residues of the mature peptide. This chemokine is a member of the CC subfamily which is characterized by two adjacent cysteine residues. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4. [provided by RefSeq, Jul 2013]
Function Chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis. [UniProt]
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Research Area Cancer kit; Cell Biology and Cellular Response kit; Immune System kit; Metabolism kit
PTM Processing at the N-terminus can regulate receptor and target cell selectivity. Deletion of the N-terminal residue converts it from an activator of basophil to an eosinophil chemoattractant.

Images (1) Click the Picture to Zoom In

  • ARG80128 Human MCP-1 / CCL2 ELISA Kit standard curve image

    ARG80128 Human MCP-1 / CCL2 ELISA Kit results of a typical standard run with optical density reading at 450 nm.

Specific References

Wnt5a promotes renal tubular inflammation in diabetic nephropathy by binding to CD146 through noncanonical Wnt signaling.

ELISA / Human / Cell culture supernatant

Xiaomei Li et al.
Cell Death Dis.,  (2021)

publication_link

 

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