ARG30263

Mitochondrial fission Antibody Duo (Drp1, BAX)

publication_link Publication4

Component

Cat No Component Name Host clonality Reactivity Application Package
ARG55200 anti-DNM1L / DRP1 antibody Rabbit pAb Hu, Ms, Rat ICC/IF, IHC-P, WB 50 μl
ARG65612 anti-Bax antibody Rabbit pAb Hu, Ms, Rat ICC/IF, IHC-P, WB 50 μl

Overview

Product Description

Mitochondria routinely undergo fission and fusion events that maintain a dynamic reticular network. Dynamin-1-like protein (DNM1L), also known as Drp1, is a GTPase that fundamentally regulates mitochondrial fission. In response to many apoptotic stimuli, oligomerization of Bax is essential for mitochondrial outer membrane permeabilization and the ensuing release of cytochrome c. These events are accompanied by mitochondrial fission that require Drp1. This antibody duo provides useful tool for the study of the mechanism of Bax oligomerization in apoptosis and other biological processes. Montessuit et al. (2010) Cell (142): 889-901

Target Name Mitochondrial fission
Alternate Names Mitochondrial fission antibody; DNM1L / DRP1 antibody; Bax antibody

Properties

Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Images (9) Click the Picture to Zoom In

  • ARG65612 anti-Bax antibody WB image

    Western blot: 30 µg of MCF-7, HCT116, U87-MG, and HepG2 cell line lysates stained with ARG65612 anti-Bax antibody at 1:500 dilution.

  • ARG65612 anti-Bax antibody ICC/IF image

    Immunocytochemistry: HeLa cells fixed with Methanol / Acetone 1:1 ratio (-20°C for 20 min) and blocked by 3% BSA in PBS (RT for 1 hour). The cells stained with ARG65612 anti-Bax antibody at 1:20 dilution (RT for 1 hour).

  • ARG55200 anti-DNM1L / DRP1 antibody ICC/IF image

    Immunofluorescence: HeLa cells stained with ARG55200 anti-DNM1L / DRP1 antibody.

  • ARG55200 anti-DNM1L / DRP1 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Rat brain tissue stained with ARG55200 anti-DNM1L / DRP1 antibody at 1:250 dilution.

  • ARG65612 anti-Bax antibody IHC-P image

    Immunohistochemistry: paraffin-embedded Human esophagus cancer tissue stained with ARG65612 anti-Bax antibody (left) at 1/30 dilution, or the same antibody preincubated with antigen (right). (Original magnification: X200)

  • ARG65612 anti-Bax antibody IHC-P image

    Immunohistochemistry: paraffin-embedded Human lung cancer tissue stained with ARG65612 anti-Bax antibody (left) at 1/30 dilution, or the same antibody preincubated with antigen (right). (Original magnification: X200)

  • ARG55200 anti-DNM1L / DRP1 antibody WB image

    Western blot: Raji cell lysate stained with ARG55200 anti-DNM1L / DRP1 antibody.

  • ARG65612 anti-Bax antibody WB image

    Western blot: 20 µg of HeLa and 3T3 cell lysates stained with ARG65612 anti-Bax antibody at 1:3000 dilution.

  • ARG55200 anti-DNM1L / DRP1 antibody WB image

    Western blot: 25 µg of Mouse brain lysate stained with ARG55200 anti-DNM1L / DRP1 antibody at 1:8000 dilution.

Specific References

Preclinical Therapeutic Assessment of a New Chemotherapeutics [Dichloro(4,4'-Bis(2,2,3,3-Tetrafluoropropoxy) Methyl)-2,2'-Bipryridine) Platinum] in an Orthotopic Patient-Derived Xenograft Model of Triple-Negative Breast Cancers

ARG65612: WB / Human

Tzu-Chun Kan et al.
Pharmaceutics.,  (2022)

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Kansuinine A Ameliorates Atherosclerosis and Human Aortic Endothelial Cell Apoptosis by Inhibiting Reactive Oxygen Species Production and Suppressing IKKβ/IκBα/NF-κB Signaling

ARG65612: WB / Human

Chen-Sheng Chen et al.
Int J Mol Sci.,  (2021)

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Multi-omics analysis of pathological changes in the amygdala of rats subjected to chronic restraint stress.

ARG65612: WB / Rat

Zhonghua Li et al.
Behav Brain Res.,  (2020)

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A LY-15, a novel cyclic pentapeptide that inhibits B16 cell proliferation and migration and induces cell apoptosis.

ARG65612: WB / Mouse

Wang Xuan et al.
Oncology Letters.,  (2018)

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