ARG82652

Mouse PDGF BB ELISA Kit

Mouse PDGF BB ELISA Kit for ELISA and Mouse

Component

Cat No Component Name Package Temp
ARG82652-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG82652-002 Standard 2 X 10 ng/vial 4°C
ARG82652-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG82652-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG82652-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG82652-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG82652-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG82652-008 25X Wash buffer 20 ml 4°C
ARG82652-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG82652-010 STOP solution 10 ml (Ready to use) 4°C
ARG82652-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG82652 Mouse PDGF BB ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse PDGF BB in serum, plasma (EDTA, heparin) and cell culture supernatants.
Tested Reactivity Ms
Tested Application ELISA
Target Name PDGF BB
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 32 pg/ml
Sample Type Serum, plasma (EDTA, heparin) and cell culture supernatants.
Standard Range 62.5 - 4000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 5.4%
Inter-Assay CV: 5.8%
Alternate Names SIS; Becaplermin; Platelet-derived growth factor subunit B; IBGC5; PDGF subunit B; c-sis; SSV; PDGF-2; Proto-oncogene c-Sis; Platelet-derived growth factor beta polypeptide; PDGF2; Platelet-derived growth factor B chain

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 18591 Mouse PDGFB

Swiss-port # P31240 Mouse Platelet-derived growth factor subunit B

Gene Symbol PDGFB
Gene Full Name platelet-derived growth factor beta polypeptide
Background This gene encodes a member of the protein family comprised of both platelet-derived growth factors (PDGF) and vascular endothelial growth factors (VEGF). The encoded preproprotein is proteolytically processed to generate platelet-derived growth factor subunit B, which can homodimerize, or alternatively, heterodimerize with the related platelet-derived growth factor subunit A. These proteins bind and activate PDGF receptor tyrosine kinases, which play a role in a wide range of developmental processes. Mutations in this gene are associated with meningioma. Reciprocal translocations between chromosomes 22 and 17, at sites where this gene and that for collagen type 1, alpha 1 are located, are associated with dermatofibrosarcoma protuberans, a rare skin tumor. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2015]
Function Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin (PubMed:26599395). Required for normal proliferation and recruitment of pericytes and vascular smooth muscle cells in the central nervous system, skin, lung, heart and placenta. Required for normal blood vessel development, and for normal development of kidney glomeruli. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFA (By similarity). [UniProt]
Cellular Localization Secreted. Note=Released by platelets upon wounding. [UniProt]
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Images (1) Click the Picture to Zoom In

  • ARG82652 Mouse PDGF BB ELISA Kit standard curve image

    ARG82652 Mouse PDGF BB ELISA Kit results of a typical standard run with optical density reading at 450 nm.

Title Download Link
ARG82652 Mouse PDGF BB ELISA Kit User manual download icon Download