ARG30034

Phospho ERBB2 / HER2 Antibody Duo (Total, pY1221/1222)

Cancer antibody; Controls and Markers antibody; Signaling Transduction antibody

Component

Cat No Component Name Host clonality Reactivity Application Package
ARG51067 anti-ERBB2 / HER2 antibody Rabbit pAb Hu ICC/IF, IHC-P, WB 50 μl
ARG51579 anti-ERBB2 / HER2 phospho (Tyr1221 / Tyr1222) antibody Rabbit pAb Hu WB, ICC/IF 50 μl

Overview

Product Description

Receptor tyrosine-protein kinase erbB-2, a 185 kDa protein, also known as CD340 (cluster of differentiation 340), proto-oncogene Neu, Erbb2 (rodent), or ERBB2 (human) is a protein that in humans is encoded by the ERBB2 gene. The ERBB2 gene is also frequently called HER2 (from human epidermal growth factor receptor 2) or HER2/neu.

HER2 is a member of the epidermal growth factor receptor (EGFR/ERBB) family. HER2 has been shown to play an important role cancer development, metastasis and chemotherapeutic resistance in many kinds of multiple cancers including, breast cancer, lung cancer, gastric cancer, ovarian cancer and so on through its overexpression or mutation. HER2 is also become an important therapeutic target for cancers.

Tyr1248 and Tyr1221/1222 are the major autophosphorylation sites on ErbB2, and the phosphorylation on these sites correct to the activity and signaling transduction of HER2.

Phosphorylation at tyrosine 1221/1222 also has been approved to improve the prediction of poor survival for breast cancer patients with hormone receptor-positive tumors. Thus, both HER2 and phosphorylated HER2 are important targets for cancer diagnosis.

Target Name ERBB2 / HER2
Alternate Names Phospho ERBB2 / HER2 antibody; Phospho erb-b2 receptor tyrosine kinase 2 antibody; Phospho ERBB2 antibody; ERBB2 / HER2 antibody; ERBB2 / HER2 phospho (Tyr1221 / Tyr1222) antibody

Properties

Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Full Name Phospho erb-b2 receptor tyrosine kinase 2 (ERBB2) Antibody Duo (Total, pY1221/1222)
Research Area Cancer antibody; Controls and Markers antibody; Signaling Transduction antibody

Images (6) Click the Picture to Zoom In

  • ARG51067 anti-HER2 antibody WB image

    Western Blot: extracts from SKOV3 and MDA cells stained with anti-HER2 antibody ARG51067.

  • ARG51579 anti-HER2 phospho (Tyr1221/Tyr1222) antibody WB image

    Western Blot: extracts from MDA cells untreated or treated with EGF stained with anti-HER2 (phospho Tyr1221/Tyr1222) antibody ARG51579.

  • ARG51579 anti-HER2 phospho (Tyr1221/Tyr1222) antibody WB image

    Western Blot: extracts from HeLa cells, treated with EGF or calf intestinal phosphatase (CIP), stained with anti-HER2 (phospho Tyr1221/Tyr1222) antibody ARG51579.

  • ARG51067 anti-HER2 antibody IHC-P image

    Immunohistochemistry: paraffin-embedded human breast carcinoma tissue stained with anti-HER2 antibody ARG51067 (left) or the same antibody preincubated with blocking peptide (right).

  • ARG51067 anti-HER2 antibody ICC/IF image

    Immunofluorescence: methanol-fixed MCF cells stained with anti-HER2 antibody ARG51067.

  • ARG51579 anti-HER2 phospho (Tyr1221/Tyr1222) antibody ICC/IF image

    Immunofluorescence: methanol-fixed MCF cells stained with anti-HER2 (phospho Tyr1221/Tyr1222) antibody ARG51579.