ARG30264

Phospho-ERK1/2 MAPK Antibody Duo (Total, pT202/Y204)

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Component

Cat No Component Name Host clonality Reactivity Application Package
ARG55797 anti-ERK1/2 antibody Rabbit pAb Hu, Ms, Rat ICC/IF, IHC-P, WB 50 μl
ARG52277 anti-ERK1/2 phospho (Thr202 / Tyr204) antibody Rabbit pAb Hu, Ms, Rat ICC/IF, IHC, WB 50 μl

Overview

Product Description

MAPKs (mitogen-activated protein kinases) are serine-threonine kinases that regulate a wide variety of cellular functions. Six groups of MAPK have so far been identified: Extracellular signal-regulated kinases (ERK1, ERK2), c-Jun N-terminal kinases (JNKs), p38 isoforms (MAPK11, MAPK12, MAPK13, MAPK14), ERK5 (MAPK7), ERK3 (MAPK6) and ERK4 (MAPK4) and ERK7/8 (MAPK15). ERK 1 and ERK 2 transduce signals from growth factors and are key in regulating differentiation and proliferation in many cell types. Upon activation by MEK, ERK1 and 2 translocate to the nucleus where they phosphorylate transcription factors such as Elk1 and downstream kinases such as p90 RSK. JNK 1,2 and 3 (sometimes known as SAPKs or stress-activated kinases). Dysregulation of MAPK kinase pathways has been associated with various diseases including cancer (ERK), neurodegeneration (JNK) and inflammation (p38). MEK1/2 catalyze the phosphorylation of human ERK1/2 at Tyr204/187 and then Thr202/185. The phosphorylation of both tyrosine and threonine is required for enzyme activation. arigo ARG30264 Erk1/2 MAPK phospho Duos contents antibodies recognize total ERK1/2 and ERK1/2 phosphorylated at Thr202/Tyr204, is useful for the researchers to study ERK1/2 phospho-cascade and monitor the total ERK1/2 expression level.

Full Name mitogen-activated protein kinase 1
Alternate Names MAPK 2; MAPK 1; MAP kinase 2; ERK-2; p41; ERK; MAP kinase 1; PRKM2; PRKM1; EC 2.7.11.24; MAPK2; p40; Extracellular signal-regulated kinase 2; p38; Mitogen-activated protein kinase 2; Mitogen-activated protein kinase 1; ERK2; MAP kinase isoform p42; p42-MAPK; P42MAPK; p41mapk; ERT1

Properties

Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Symbol MAPK1
Gene Full Name mitogen-activated protein kinase 1
Background This gene encodes a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. One study also suggests that this protein acts as a transcriptional repressor independent of its kinase activity. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene. [provided by RefSeq, Jan 2014]
Function Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade plays also a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. [UniProt]
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PTM Phosphorylated upon KIT and FLT3 signaling (By similarity). Dually phosphorylated on Thr-185 and Tyr-187, which activates the enzyme. Undergoes regulatory phosphorylation on additional residues such as Ser-246 and Ser-248 in the kinase insert domain (KID) These phosphorylations, which are probably mediated by more than one kinase, are important for binding of MAPK1/ERK2 to importin-7 (IPO7) and its nuclear translocation. In addition, autophosphorylation of Thr-190 was shown to affect the subcellular localization of MAPK1/ERK2 as well. Ligand-activated ALK induces tyrosine phosphorylation. Dephosphorylated by PTPRJ at Tyr-187. Phosphorylation on Ser-29 by SGK1 results in its activation by enhancing its interaction with MAP2K1/MEK1 and MAP2K2/MEK2. DUSP3 and DUSP6 dephosphorylate specifically MAPK1/ERK2 and MAPK3/ERK1 whereas DUSP9 dephosphorylates a broader range of MAPKs.
ISGylated.

Images (6) Click the Picture to Zoom In

  • ARG52277 & ARG55797 WB image

    Western blot: 30 µg of 1) HeLa, 2) HeLa + EGF 5 min, and 3) HeLa + EGF 10 min stained with ARG52277 anti-ERK1/2 phospho (Thr202 / Tyr204) antibody (Top) and ARG55797 anti-ERK1/2 antibody (Bottom) at 1:1000 dilution.

  • ARG55797 anti-ERK1/2 antibody ICC/IF image

    Immunofluorescence: A549 cell stained with ARG55797 anti-ERK1/2 antibody. Blue: DAPI for nuclear staining.

  • ARG55797 anti-ERK1/2 antibody WB image

    Western blot: 30 µg of Jurkat, Mouse brain, and Rat brain lysates stained with ARG55797 anti-ERK1/2 antibody at 1:1000 dilution.

  • ARG52277 anti-ERK1/2 phospho (Thr202 / Tyr204) antibody WB image

    Western blot: 30 µg of HeLa cells untreated or treated with TNF-alpha at 20 ng/ml dilution (10, 20, and 30 min). The blots were stained with ARG52277 anti-ERK1/2 phospho (Thr202 / Tyr204) antibody at 1:1000 dilution.

  • ARG55797 anti-ERK1/2 antibody ICC/IF image

    Immunofluorescence: A549 cells stained with ARG55797 anti-ERK1/2 antibody.

  • ARG55797 anti-ERK1/2 antibody WB image

    Western blot: 30 µg of Rat spleen lysate stained with ARG55797 anti-ERK1/2 antibody at 1:500 dilution.

Specific References

Four new antitumor metabolites isolated from a mutant 3-f-31 strain derived from Penicillium purpurogenum G59.

WB / Human

Wang Nan et al.
Eur J Med Chem.,  (2018)

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