ARG30247

Phospho eNOS Antibody Duo (Total, pY1177)

Cancer antibody; Cell Biology and Cellular Response antibody; Metabolism antibody; Neuroscience antibody
publication_link Publication1

Component

Cat No Component Name Host clonality Reactivity Application Package
ARG20524 anti-eNOS phospho (Ser1177) antibody Rabbit pAb Hu, Ms, Rat ELISA, WB 50 μl
ARG53368 anti-eNOS antibody Rabbit pAb Hu, Ms, Rat ELISA, ICC/IF, IHC-P, IHC-Fr, IP, WB 250 μl

Overview

Target Name eNOS
Alternate Names Phospho eNOS antibody; Phospho Endothelial NOS antibody; eNOS phospho (Ser1177) antibody; eNOS antibody

Properties

Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Symbol NOS3
Gene Full Name Phospho Endothelial NOS (eNOS) Antibody Duo
Background Nitric oxide (NO) has a broad range of biological activities and is implicated in signaling pathways in phylogenetically diverse species. Nitric oxide synthases (NOS), the enzymes responsible for synthesis of NO, are homodimers whose monomers are themselves two fused enzymes: a cytochrome reductase and a cytochrome that requires three cosubstrates (L-arginine, NADPH, and oxygen) and five cofactors or prosthetic groups (FAD, FMN, calmodulin, tetrahydrobiopterin, and heme). Several distinct NOS isoforms are produced from three distinct genes, inducible NOS (iNOS, NOS-II), neuronal NOS (bNOS, NOS-I), and endothelial NOS (eNOS, ecNOS, NOS-III). Regulation of eNOS activity occurs through phosphorylation at multiple sites. Phosphorylation of Ser-633 (mouse Ser-632) in the FMN binding domain increases eNOS activity and may be important for the maintenance of NO synthesis after initial activation by Ca2+ flux and Ser-1177 phosphorylation. Tyr-657 is phosphorylated by PYK2 in response to fluid shear stress and this phosphorylation leads to attenuation of enzyme activity.
Function Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.
Isoform eNOS13C: Lacks eNOS activity, dominant-negative form that may down-regulate eNOS activity by forming heterodimers with isoform 1. [UniProt]
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Metabolism antibody; Neuroscience antibody
PTM Phosphorylation by AMPK at Ser-1177 in the presence of Ca(2+)-calmodulin (CaM) activates activity. In absence of Ca(2+)-calmodulin, AMPK also phosphorylates Thr-495, resulting in inhibition of activity (By similarity). Phosphorylation of Ser-114 by CDK5 reduces activity.

Images (2) Click the Picture to Zoom In

  • ARG20524 anti-eNOS phospho (Ser1177) antibody WB image

    Western Blot: 1) and 2) calyculin A (100 nM) treated human umbilical vein endothelial cells for 30 min; 2) then the blots were treated with lambda phosphatase. The blots were stained with ARG20524 anti-eNOS phospho (Ser1177) antibody.

  • ARG53368 anti-Nitric Oxide Synthase-Endothelial (eNOS) antibody IHC-P image

    Immunohistochemistry: Human Placenta stained with Nitric Oxide Synthase-Endothelial (eNOS) antibody (ARG53368)

Specific References

Isolation and Characterization of Three Anti-hypertension Peptides from the Mycelia of Ganoderma Lucidum (Agaricomycetes).

ARG20524: WB / Human

Wu Qiang et al.
J Agric Food Chem.,  (2019)

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