ARG82372

Rat PDGF BB ELISA Kit

Rat PDGF BB ELISA Kit for ELISA and Rat

Component

Cat No Component Name Package Temp
ARG82372-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG82372-002 Standard 2 X 10 ng/vial 4°C
ARG82372-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG82372-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG82372-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG82372-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG82372-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG82372-008 25X Wash buffer 20 ml 4°C
ARG82372-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG82372-010 STOP solution 10 ml (Ready to use) 4°C
ARG82372-011 Plate sealer 4 strips Room temperature

Overview

Product Description ARG82372 Rat PDGF BB ELISA Kit is an Enzyme Immunoassay kit for the quantification of Rat PDGF BB in serum, plasma (EDTA, heparin) and cell culture supernatants.
Tested Reactivity Rat
Tested Application ELISA
Target Name PDGF BB
Conjugation HRP
Conjugation Note Substrate: TMB and read at 450 nm.
Sensitivity 31.25 pg/ml
Sample Type Serum, plasma (EDTA, heparin) and cell culture supernatants.
Standard Range 62.5 - 4000 pg/ml
Sample Volume 100 µl
Precision Intra-Assay CV: 4.9%
Inter-Assay CV: 7.2%
Alternate Names SIS; Becaplermin; Platelet-derived growth factor subunit B; IBGC5; PDGF subunit B; c-sis; SSV; PDGF-2; Proto-oncogene c-Sis; Platelet-derived growth factor beta polypeptide; PDGF2; Platelet-derived growth factor B chain

Application Instructions

Assay Time ~ 5 hours

Properties

Form 96 well
Storage Instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 24628 Rat PDGFB

Swiss-port # Q05028 Rat Platelet-derived growth factor subunit B

Gene Symbol PDGFB
Gene Full Name platelet-derived growth factor beta polypeptide
Background The protein encoded by this gene is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a motif of eight cysteines. This gene product can exist either as a homodimer (PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha polypeptide (PDGF-AB), where the dimers are connected by disulfide bonds. Mutations in this gene are associated with meningioma. Reciprocal translocations between chromosomes 22 and 17, at sites where this gene and that for collagen type 1, alpha 1 are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans resulting from unregulated expression of growth factor. Two alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Feb 2013]
Function Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal proliferation and recruitment of pericytes and vascular smooth muscle cells in the central nervous system, skin, lung, heart and placenta. Required for normal blood vessel development, and for normal development of kidney glomeruli. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFA (By similarity). [UniProt]
Cellular Localization Secreted. Note=Released by platelets upon wounding. [UniProt]
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Images (1) Click the Picture to Zoom In

  • ARG82372 Rat PDGF BB ELISA Kit standard curve image

    ARG82372 Rat PDGF BB ELISA Kit results of a typical standard run with optical density reading at 450 nm.

Title Download Link
ARG82372 Rat PDGF BB ELISA kit User manual download icon Download