ARG54387

anti-AIF antibody

anti-AIF antibody for ICC/IF,Western blot and Human

Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Gene Regulation antibody; Metabolism antibody
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Overview

Product Description

Rabbit Polyclonal antibody recognizes AIF

Tested Reactivity Hu
Tested Application ICC/IF, WB
Specificity This antibody recognizes human, mouse, and rat AIF (67 kD).
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name AIF
Antigen Species Human
Immunogen Peptide corresponding to aa 593-606 at the C-terminus of human AIF. This sequence is identical to those of mouse and rat AIF.
Conjugation Un-conjugated
Alternate Names CMTX4; NAMSD; COWCK; Apoptosis-inducing factor 1, mitochondrial; CMT2D; EC 1.1.1.-; NADMR; PDCD8; COXPD6; AIF; Programmed cell death protein 8

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IFAssay-dependent
WBAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control K562

Properties

Form Liquid
Purification Immunoaffinity chroma-tography
Buffer PBS (pH 7.4) and 0.02% Sodium azide
Preservative 0.02% Sodium azide
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 9131 Human AIFM1

Swiss-port # O95831 Human Apoptosis-inducing factor 1, mitochondrial

Gene Symbol AIFM1
Gene Full Name apoptosis-inducing factor, mitochondrion-associated, 1
Background A novel protein that causes chromatin condensation and DNA fragmentation has been designated Apoptosis Inducing Factor (AIF). AIF localizes in mitochondria and translocates to the nucleus when apoptosis is induced. This event is followed by the release of chytochrome c and caspase-9 from mitochondria. AIF is highly conserved between human and mouse and widely expressed in different cell types and tissues.
Function Functions both as NADH oxidoreductase and as regulator of apoptosis. In response to apoptotic stimuli, it is released from the mitochondrion intermembrane space into the cytosol and to the nucleus, where it functions as a proapoptotic factor in a caspase-independent pathway. In contrast, functions as an antiapoptotic factor in normal mitochondria via its NADH oxidoreductase activity. The soluble form (AIFsol) found in the nucleus induces 'parthanatos' i.e. caspase-independent fragmentation of chromosomal DNA. Interacts with EIF3G,and thereby inhibits the EIF3 machinery and protein synthesis, and activates casapse-7 to amplify apoptosis. Plays a critical role in caspase-independent, pyknotic cell death in hydrogen peroxide-exposed cells. Binds to DNA in a sequence-independent manner. [UniProt]
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Gene Regulation antibody; Metabolism antibody
Calculated MW 67 kDa
PTM Under normal conditions, a 54-residue N-terminal segment is first proteolytically removed during or just after translocation into the mitochondrial intermembrane space (IMS) by the mitochondrial processing peptidase (MPP) to form the inner-membrane-anchored mature form (AIFmit). During apoptosis, it is further proteolytically processed at amino-acid position 101 leading to the generation of the mature form, which is confined to the mitochondrial IMS in a soluble form (AIFsol). AIFsol is released to the cytoplasm in response to specific death signals, and translocated to the nucleus, where it induces nuclear apoptosis in a caspase-independent manner.
Ubiquitination by XIAP/BIRC4 does not lead to proteasomal degradation. Ubiquitination at Lys-255 by XIAP/BIRC4 blocks its ability to bind DNA and induce chromatin degradation, thereby inhibiting its ability to induce cell death.

Images (2) Click the Picture to Zoom In

  • ARG54387 anti-AIF antibody WB image

    Western blot: 30 µg of 293T cell lysate stained with ARG54387 anti-AIF antibody at 1:500 dilution.

  • ARG54387 anti-AIF antibody ICC/IF image

    Immunofluorescence: K562 stained with ARG54387 anti-AIF antibody at 5 μg/ml dilution.

Customer's Feedback

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Review for anti-AIF antibody

Application:WB

Sample:MCF-7

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:500

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC