ARG54404

anti-APAF1 antibody

anti-APAF1 antibody for Western blot,Immunohistochemistry and Human

Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes APAF1
Tested Reactivity Hu
Tested Application IHC, WB
Specificity This antibody recognizes full-length mouse and human Apaf1 (115-130kDa).
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name APAF1
Antigen Species Human
Immunogen Peptide corresponding to aa 12-28 of human Apaf1. This sequence is identical in human and mouse.
Conjugation Un-conjugated
Alternate Names CED4; APAF-1; Apoptotic protease-activating factor 1

Application Instructions

Application Suggestion
Tested Application Dilution
IHCAssay-dependent
WBAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control HeLa and K562

Properties

Form Liquid
Purification Immunoaffinity chroma-tography
Buffer PBS (pH 7.4) and 0.02% Sodium azide
Preservative 0.02% Sodium azide
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 317 Human APAF1

Swiss-port # O14727 Human Apoptotic protease-activating factor 1

Gene Symbol APAF1
Gene Full Name apoptotic peptidase activating factor 1
Background The mammalian homologues of the key cell death gene CED-4 in C. elegans has been identified recently from human and mouse and designated Apaf1 (for apoptosis protease-activating factor 1). Apaf1 binds to cytochrome c (Apaf2) and caspase-9 (Apaf3), which leads to caspase-9 activation. Activated caspase-9 in turn cleaves and activates caspase-3, one of the key proteases responsible for the proteolytic cleavage of many key proteins in apoptosis. Apaf1 can also associate with caspase-4 and caspase-8. Apaf1 is ubiquitously expressed in human tissues.
Function Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis. [UniProt]
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody
Calculated MW 142 kDa

Images (2) Click the Picture to Zoom In

  • ARG54404 anti-APAF1 antibody WB image

    Western blot: K562 cell lysate in the (A) absence and (B) presence of blocking peptide. stained with ARG54404 anti-APAF1 antibody at 1 μg/ml dilution.

  • ARG54404 anti-APAF1 antibody IHC image

    Immunohistochemistry: Human heart tissue stained with ARG54404 anti-APAF1 antibody at 1 μg/ml dilution.