anti-ATG4A antibody

anti-ATG4A antibody for Western blot and Human

Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody


Product Description Mouse Monoclonal antibody recognizes ATG4A
Tested Reactivity Hu
Tested Application WB
Host Mouse
Clonality Monoclonal
Clone 1458CT808.66.25.69
Isotype IgG2b, kappa
Target Name ATG4A
Conjugation Un-conjugated
Alternate Names Cysteine protease ATG4A; Autophagin-2; AUT-like 2 cysteine endopeptidase; APG4A; Autophagy-related cysteine endopeptidase 2; EC 3.4.22.-; AUTL2; Autophagy-related protein 4 homolog A; hAPG4A

Application Instructions

Application Suggestion
Tested Application Dilution
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control K562


Form Liquid
Purification Purification with Protein G.
Buffer PBS and 0.09% (W/V) Sodium azide
Preservative 0.09% (W/V) Sodium azide
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 115201 Human ATG4A

Swiss-port # Q8WYN0 Human Cysteine protease ATG4A

Gene Symbol ATG4A
Gene Full Name autophagy related 4A, cysteine peptidase
Background Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. Transcript variants that encode distinct isoforms have been identified. [provided by RefSeq, Jul 2008]
Function Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Preferred substrate is GABARAPL2 followed by MAP1LC3A and GABARAP. Has also an activity of delipidating enzyme for the PE-conjugated forms. [UniProt]
Cellular Localization Cytoplasm.
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody
Calculated MW 45 kDa

Images (1) Click the Picture to Zoom In

  • ARG54817 anti-ATG4A antibody WB image

    Western blot: 20 µg of K562 cell lysate stained with ARG54817 anti-ATG4A antibody at 1:500 dilution.