ARG54406

anti-BIM antibody

anti-BIM antibody for Western blot,ICC/IF and Human

Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Immune System antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes BIM
Tested Reactivity Hu
Tested Application ICC/IF, WB
Specificity This antibody recognizes full-length Bim (23kDa) of human, mouse, and rat.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name BIM
Antigen Species Human
Immunogen Peptide corresponding to aa 22-40 of human Bim. The sequence is identical to that of mouse and is different by one amino acid from that of rat.
Conjugation Un-conjugated
Alternate Names Bcl2-L-11; BIM; Bcl2-interacting mediator of cell death; Bcl-2-like protein 11; BAM; BOD

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IFAssay-dependent
WBAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control K562 and A549

Properties

Form Liquid
Purification Immunoaffinity chroma-tography
Buffer PBS (pH 7.4) and 0.02% Sodium azide
Preservative 0.02% Sodium azide
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 10018 Human BCL2L11

Swiss-port # O43521 Human Bcl-2-like protein 11

Gene Symbol BCL2L11
Gene Full Name BCL2-like 11 (apoptosis facilitator)
Background A novel BH3 domain-containing protein was recently identified and designated Bim or BOD from human, mouse, and rat. Bim/BOD interacts with diverse members in the pro-survival Bcl-2 subfamily including Bcl-2, Bcl-x L , and Bcl-w. Bim/BOD induces apoptosis. The messenger RNA of Bim is ubiquitously expressed in multiple tissues and cell lines.
Function Induces apoptosis and anoikis. Isoform BimL is more potent than isoform BimEL. Isoform Bim-alpha1, isoform Bim-alpha2 and isoform Bim-alpha3 induce apoptosis, although less potent than isoform BimEL, isoform BimL and isoform BimS. Isoform Bim-gamma induces apoptosis. Isoform Bim-alpha3 induces apoptosis possibly through a caspase-mediated pathway. Isoform BimAC and isoform BimABC lack the ability to induce apoptosis. [UniProt]
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Immune System antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody
Calculated MW 22 kDa
PTM Phosphorylation at Ser-69 by MAPK1/MAPK3 leads to interaction with TRIM2 and polyubiquitination, followed by proteasomal degradation (PubMed:15486195, PubMed:21478148). Deubiquitination catalyzed by USP27X stabilizes the protein (By similarity).
Ubiquitination by TRIM2 following phosphorylation by MAPK1/MAPK3 leads to proteasomal degradation. Conversely, deubiquitination catalyzed by USP27X stabilizes the protein.

Images (2) Click the Picture to Zoom In

  • ARG54406 anti-BIM antibody WB image

    Western blot: K:K562; A:A549 stained with ARG54406 anti-BIM antibody at 1 μg/ml dilution.

  • ARG54406 anti-BIM antibody IHC image

    Immunohistochemistry: Human skin cancer cells stained with ARG54406 anti-BIM antibody at 20 μg/ml dilution.