ARG21418

anti-Bax antibody [2D2]

anti-Bax antibody [2D2] for Western blot,Immunoprecipitation,Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,ICC/IF,Neutralizing,Purification and Human,Monkey,Dog,Bovine

Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Mitochondrial fission antibody; Apoptosis Marker antibody; Pro-apoptotic Bcl2 protein antibody

Overview

Product Description Mouse Monoclonal antibody [2D2] recognizes Bax
Tested Reactivity Hu, Bov, Dog, Mk
Tested Application FACS, ICC/IF, IHC-P, IP, Neut, Puri, WB
Specificity Human/Monkey/Canine/Bovine Bax. The clone 2D2 reacts with an N-terminal epitope between amino acids 3-16 of human Bax. It can bind both soluble and detergent-altered forms of Bax as well as Bax-Bcl-2 and Bax-Bcl-xL heterodimers.
Host Mouse
Clonality Monoclonal
Clone 2D2
Isotype IgG1, kappa
Target Name Bax
Antigen Species Human
Immunogen Human Bax peptide (a.a. 3-16)
Conjugation Un-conjugated
Alternate Names Bcl-2-like protein 4; Bcl2-L-4; BCL2L4; Apoptosis regulator BAX

Application Instructions

Application Suggestion
Tested Application Dilution
FACSAssay-dependent
ICC/IFAssay-dependent
IHC-PAssay-dependent
IPAssay-dependent
NeutAssay-dependent
PuriAssay-dependent
WB< 2 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Buffer BBS (pH 8.2)
Concentration 0.1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 280730 Bovine BAX

GeneID: 581 Human BAX

Swiss-port # O02703 Bovine Apoptosis regulator BAX

Swiss-port # Q07812 Human Apoptosis regulator BAX

Gene Symbol BAX
Gene Full Name BCL2-associated X protein
Background Bax belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. The association and the ratio of BAX to BCL2 also determines survival or death of a cell following an apoptotic stimulus. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for this gene. [provided by RefSeq, Dec 2019]
Function Bax plays a role in the mitochondrial apoptotic process. Under normal conditions, BAX is largely cytosolic via constant retrotranslocation from mitochondria to the cytosol mediated by BCL2L1/Bcl-xL, which avoids accumulation of toxic BAX levels at the mitochondrial outer membrane (MOM) (PubMed:21458670). Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis. [UniProt]
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Mitochondrial fission antibody; Apoptosis Marker antibody; Pro-apoptotic Bcl2 protein antibody
Calculated MW 21 kDa

Images (1) Click the Picture to Zoom In

  • ARG21418 anti-Bax antibody [2D2] WB image

    Western blot: HL60 total cell lysate stained with ARG21418 anti-Bax antibody [2D2]. Secondary antibodies: ARG21886 Goat anti-Mouse IgG1 antibody (HRP), pre-adsorbed.