ARG54331

anti-CAD antibody

anti-CAD antibody for Western blot,Immunohistochemistry and Mouse

Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Gene Regulation antibody; Metabolism antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes CAD
Tested Reactivity Ms
Tested Application IHC, WB
Specificity This antibody recognizes full-length mouse CAD (40kDa).
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name CAD
Antigen Species Mouse
Immunogen Peptide corresponding to aa 314-329 of mouse CAD (accession no. O54788).
Conjugation Un-conjugated
Alternate Names Caspase-activated DNase; DFF40; DFF-40; Caspase-activated deoxyribonuclease; DNA fragmentation factor 40 kDa subunit; Caspase-activated nuclease; CPAN; DNA fragmentation factor subunit beta; EC 3.-.-.-; DFF2; CAD

Application Instructions

Application Suggestion
Tested Application Dilution
IHCAssay-dependent
WBAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Mouse lung and kidney

Properties

Form Liquid
Purification Immunoaffinity chroma-tography
Buffer PBS (pH 7.4) and 0.02% Sodium azide
Preservative 0.02% Sodium azide
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 13368 Mouse DFFB

Swiss-port # O54788 Mouse DNA fragmentation factor subunit beta

Gene Symbol Dffb
Gene Full Name DNA fragmentation factor, beta subunit
Background Cell death signals are transduced by death domain-containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A mouse DNase that causes DNA fragmentation was identified recently and designated CAD (caspase activated deoxyribo-nuclease). Activation of CAD/DFF40, which causes DNA degradation, is the hallmark of apoptotic cell death.
Function Nuclease that induces DNA fragmentation and chromatin condensation during apoptosis. Degrades naked DNA and induces apoptotic morphology. [UniProt]
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Gene Regulation antibody; Metabolism antibody
Calculated MW 39 kDa

Images (2) Click the Picture to Zoom In

  • ARG54331 anti-CAD antibody WB image

    Western Blot: murine lung (L) and kidney (K) tissue lysates stained with ARG54331 anti-CAD antibody at 1 μg/ml dilution.

  • ARG54331 anti-CAD antibody IHC image

    Immunohistochemistry: mouse kidney tissue stained with ARG54331 anti-CAD antibody at 1 μg/ml dilution.