ARG54436

anti-CARD9 antibody

anti-CARD9 antibody for Western blot,ICC/IF and Human

Cell Biology and Cellular Response antibody; Cell Death antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes CARD9
Tested Reactivity Hu
Tested Application ICC/IF, WB
Specificity This antibody recognizes human CARD9, a novel CARD (Caspase Recruitment Domain)-containing protein that interacts with the CARD activation domain of Bcl-10. CARD9 associates with Bcl-10 to form a complex within cells. CARD9 induces apoptosis and activates NF- B.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name CARD9
Antigen Species Human
Immunogen Peptide corresponding to aa 521-536 of human CARD9 (accession no. AF31187). This sequence differs from that of rat CARD9 by two amino acids.
Conjugation Un-conjugated
Alternate Names CANDF2; hCARD9; Caspase recruitment domain-containing protein 9

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IFAssay-dependent
WBAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control MB-361, PC-3 and K562

Properties

Form Liquid
Purification Immunoaffinity chroma-tography
Buffer PBS (pH 7.4) and 0.02% Sodium azide
Preservative 0.02% Sodium azide
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 64170 Human CARD9

Swiss-port # Q9H257 Human Caspase recruitment domain-containing protein 9

Gene Symbol CARD9
Gene Full Name caspase recruitment domain family, member 9
Background The protein encoded by this gene is a member of the CARD protein family, which is defined by the presence of a characteristic caspase-associated recruitment domain (CARD). CARD is a protein interaction domain known to participate in activation or suppression of CARD containing members of the caspase family, and thus plays an important regulatory role in cell apoptosis. This protein was identified by its selective association with the CARD domain of BCL10, a postive regulator of apoptosis and NF-kappaB activation, and is thought to function as a molecular scaffold for the assembly of a BCL10 signaling complex that activates NF-kappaB. Several alternatively spliced transcript variants have been observed, but their full-length nature is not clearly defined. [provided by RefSeq, Jul 2008]
Function Adapter protein that plays a key role in innate immune response to a number of intracellular pathogens, such as C.albicans and L.monocytogenes. Is at the crossroads of ITAM-tyrosine kinase and the Toll-like receptors (TLR) and NOD2 signaling pathways. Probably controls various innate immune response pathways depending on the intracellular pathogen. In response to L.monocytogenes infection, acts by connecting NOD2 recognition of peptidoglycan to downstream activation of MAP kinases (MAPK) without activating NF-kappa-B. Also involved in activation of myeloid cells via classical ITAM-associated receptors and TLR: required for TLR-mediated activation of MAPK, while it is not required for TLR-induced activation of NF-kappa-B (By similarity). Controls CLEC7A (dectin-1)-mediated myeloid cell activation induced by the yeast cell wall component zymosan, leading to cytokine production and innate anti-fungal immunity: acts by regulating BCL10-MALT1-mediated NF-kappa-B activation pathway. Activates NF-kappa-B via BCL10. In response to the hyphal form of C.albicans, mediates CLEC6A (dectin-2)-induced I-kappa-B kinase ubiquitination, leading to NF-kappa-B activation via interaction with BCL10. In response to fungal infection, may be required for the development and subsequent differentiation of interleukin 17-producing T helper (TH-17) cells. [UniProt]
Research Area Cell Biology and Cellular Response antibody; Cell Death antibody
Calculated MW 62 kDa
PTM Phosphorylated at Thr-531 and Thr-533 by CK2 following interaction with VHL, leading to inhibit the ability to activate NF-kappa-B.

Images (2) Click the Picture to Zoom In

  • ARG54436 anti-CARD9 antibody WB image

    Western Blot: A:MDA-MB-361; B:PC-3 stained with ARG54436 anti-CARD9 antibody at 2.5 μg/ml dilution.

  • ARG54436 anti-CARD9 antibody ICC/IF image

    Immunofluorescence: K562 stained with ARG54436 anti-CARD9 antibody at 10 μg/ml dilution.