ARG20805

anti-CD106 / VCAM1 antibody [M/K-2] (azide free)

anti-CD106 / VCAM1 antibody [M/K-2] (azide free) for Flow cytometry,IHC-Frozen sections,Immunoprecipitation,Western blot,Blocking and Mouse

Overview

Product Description Azide free and low endotoxin Rat Monoclonal antibody [M/K-2] recognizes CD106 / VCAM1
Tested Reactivity Ms
Tested Application BL, FACS, IHC-Fr, IP, WB
Specificity Mouse CD106. The clone MK-2 has been used in transplant studies to suppress cardiac rejection and induce long-term cardiac graft survival. The M/K-2 monoclonal antibody immunoprecipitates a peptide that gives a single band on SDS-PAGE gels with an apparent MW of ~100 kDa under reducing conditions and 92 kDa under non-reducing conditions.
Host Rat
Clonality Monoclonal
Clone M/K-2
Isotype IgG1, kappa
Target Name CD106 / VCAM1
Antigen Species Mouse
Immunogen BALB/3T3 and +/+2.4 cells
Conjugation Un-conjugated
Full Name vascular cell adhesion molecule 1
Alternate Names CD106; INCAM-100; Vascular cell adhesion protein 1; VCAM-1; CD antigen CD106; V-CAM 1

Application Instructions

Application Suggestion
Tested Application Dilution
BLAssay-dependent
FACSAssay-dependent
IHC-FrAssay-dependent
IPAssay-dependent
WBAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 81 kDa

Properties

Form Liquid
Purification Note Low endotoxin
Buffer PBS
Concentration 0.5 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 22329 Mouse VCAM1

Swiss-port # P29533 Mouse Vascular cell adhesion protein 1

Gene Symbol VCAM1
Gene Full Name vascular cell adhesion molecule 1
Background This gene is a member of the Ig superfamily and encodes a cell surface sialoglycoprotein expressed by cytokine-activated endothelium. This type I membrane protein mediates leukocyte-endothelial cell adhesion and signal transduction, and may play a role in the development of artherosclerosis and rheumatoid arthritis. Three alternatively spliced transcripts encoding different isoforms have been described for this gene. [provided by RefSeq, Dec 2010]
Function Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with integrin alpha-4/beta-1 (ITGA4/ITGB1) on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/ITGA4/ITGB1 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation. [UniProt]
PTM Sialoglycoprotein.