ARG20914

anti-CD16/32 antibody [93] (azide free)

anti-CD16/32 antibody [93] (azide free) for Flow cytometry,IHC-Frozen sections,Immunoprecipitation,Blocking and Mouse

Developmental Biology antibody; Immune System antibody; General Lymphocyte Marker Study antibody; Natural killer cells antibody

Overview

Product Description Azide free and low endotoxin Rat Monoclonal antibody [93] recognizes CD16/32
Tested Reactivity Ms
Tested Application BL, FACS, IHC-Fr, IP
Specificity Mouse CD16/32. The clone 93 recognizes a conformational epitope formed by FcγII and FcγIII receptors.
Host Rat
Clonality Monoclonal
Clone 93
Isotype IgG2a, lambda
Target Name CD16/32
Species Mouse
Immunogen VH81X Tg B cells and T220 stromal cell line
Conjugation Un-conjugated
Full Name Fc fragment of IgG, low affinity IIIa, receptor (CD16a)
Alternate Names FCRIIIA; FcRIIIa; CD antigen CD16a; Fc-gamma RIII-alpha; FCR-10; FcR-10; FCRIII; FCG3; Low affinity immunoglobulin gamma Fc region receptor III-A; FCGRIII; CD16; Fc-gamma RIIIa; IgG Fc receptor III-2; IMD20; CD16A; IGFR3; CD16a antigen; FCGR3; FcRIII; Fc-gamma RIII

Application Instructions

Application Suggestion
Tested Application Dilution
BLAssay-dependent
FACS< 3 µg/10^6 cells
IHC-FrAssay-dependent
IPAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 29 kDa

Properties

Form Liquid
Purification Note Low endotoxin
Buffer PBS
Concentration 0.5 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Symbol FCGR3A
Gene Full Name Fc fragment of IgG, low affinity IIIa, receptor (CD16a)
Background This gene encodes a receptor for the Fc portion of immunoglobulin G, and it is involved in the removal of antigen-antibody complexes from the circulation, as well as other other antibody-dependent responses. This gene (FCGR3A) is highly similar to another nearby gene (FCGR3B) located on chromosome 1. The receptor encoded by this gene is expressed on natural killer (NK) cells as an integral membrane glycoprotein anchored through a transmembrane peptide, whereas FCGR3B is expressed on polymorphonuclear neutrophils (PMN) where the receptor is anchored through a phosphatidylinositol (PI) linkage. Mutations in this gene have been linked to susceptibility to recurrent viral infections, susceptibility to systemic lupus erythematosus, and alloimmune neonatal neutropenia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Function Receptor for the Fc region of IgG. Binds complexed or aggregated IgG and also monomeric IgG. Mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis. [UniProt]
Highlight Related products:
CD16 antibodies; CD16 ELISA Kits; CD16 Duos / Panels; Anti-Rat IgG secondary antibodies;
Related news:
Tumor-Infiltrating Lymphocytes (TILs)
Research Area Developmental Biology antibody; Immune System antibody; General Lymphocyte Marker Study antibody; Natural killer cells antibody
PTM Glycosylated. Contains high mannose- and complex-type oligosaccharides. Glycosylation at Asn-180 is mandatory for high affinity binding to the Fc and for discrimination between fucosylated and afucosylated IgG glycoforms.
The soluble form is produced by a proteolytic cleavage.