anti-CD29 / Integrin beta 1 antibody [JB1B]

anti-CD29 / Integrin beta 1 antibody [JB1B] for IHC-Frozen sections,ELISA,Flow cytometry,Immunoprecipitation,Western blot and Human,Pig,Rhesus Monkey


Product Description Mouse Monoclonal antibody [JB1B] recognizes CD29 / Integrin beta 1
Mouse anti Human CD29 antibody, clone JB1B recognizes human integrin beta1, also known as CD29, Fibronectin receptor subunit beta, Glycoprotein IIa or VLA-4 subunit beta. CD29 is a 798 amino acid ~130 kDa single pass type-1 transmembrane glycoprotein containing a single VWFA domain. Specificity for CD29 has been confirmed with integrin beta1 transfectants and by reciprocal preclearing of beta1 reactivity with anti-beta1 antibody.Mouse anti Human CD29 antibody, clone JB1B has been successfully employed for the detection of porcine CD29 in formalin fixed, paraffin embedded material (Papageorgiou et al. 2014) and also to stimulate the adherence of several cell types (Wilkins et al. 1996).The removal of azide is recommended prior to use in functional assays.
Tested Reactivity Hu, Pig, R. Mk
Species Does Not React With Rat
Tested Application ELISA, FACS, IHC-Fr, IP, WB
Host Mouse
Clonality Monoclonal
Clone JB1B
Isotype IgG2a
Target Name CD29 / Integrin beta 1
Antigen Species Human
Immunogen Purified Human beta1 integrin
Conjugation Un-conjugated
Alternate Names CD29; Glycoprotein IIa; Fibronectin receptor subunit beta; VLAB; MSK12; CD antigen CD29; FNRB; GPIIA; VLA-4 subunit beta; VLA-BETA; MDF2; Integrin beta-1

Application Instructions

Application Suggestion
Tested Application Dilution
ELISA0.1 - 1 µg/ml
FACS1 µg/10^6 cells
IHC-Fr5 µg/ml
IP1 - 5 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.


Form Liquid
Purification Purified by ammonium sulfate precipitation
Buffer PBS and 0.09% Sodium azide
Preservative 0.09% Sodium azide
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 3688 Human ITGB1

GeneID: 397019 Pig ITGB1

Swiss-port # P05556 Human Integrin beta-1

Swiss-port # Q9GLP0 Pig Integrin beta-1

Gene Symbol ITGB1
Gene Full Name integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12)
Background Integrins are heterodimeric proteins made up of alpha and beta subunits. At least 18 alpha and 8 beta subunits have been described in mammals. Integrin family members are membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metastatic diffusion of tumor cells. This gene encodes a beta subunit. Multiple alternatively spliced transcript variants which encode different protein isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Function Integrins alpha-1/beta-1, alpha-2/beta-1, alpha-10/beta-1 and alpha-11/beta-1 are receptors for collagen. Integrins alpha-1/beta-1 and alpha-2/beta-2 recognize the proline-hydroxylated sequence G-F-P-G-E-R in collagen. Integrins alpha-2/beta-1, alpha-3/beta-1, alpha-4/beta-1, alpha-5/beta-1, alpha-8/beta-1, alpha-10/beta-1, alpha-11/beta-1 and alpha-V/beta-1 are receptors for fibronectin. Alpha-4/beta-1 recognizes one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. Integrin alpha-5/beta-1 is a receptor for fibrinogen. Integrin alpha-1/beta-1, alpha-2/beta-1, alpha-6/beta-1 and alpha-7/beta-1 are receptors for lamimin. Integrin alpha-4/beta-1 is a receptor for VCAM1. It recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-9/beta-1 is a receptor for VCAM1, cytotactin and osteopontin. It recognizes the sequence A-E-I-D-G-I-E-L in cytotactin. Integrin alpha-3/beta-1 is a receptor for epiligrin, thrombospondin and CSPG4. Alpha-3/beta-1 may mediate with LGALS3 the stimulation by CSPG4 of endothelial cells migration. Integrin alpha-V/beta-1 is a receptor for vitronectin. Beta-1 integrins recognize the sequence R-G-D in a wide array of ligands. Isoform 2 interferes with isoform 1 resulting in a dominant negative effect on cell adhesion and migration (in vitro). In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. When associated with alpha-7/beta-1 integrin, regulates cell adhesion and laminin matrix deposition. Involved in promoting endothelial cell motility and angiogenesis. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process and the formation of mineralized bone nodules. May be involved in up-regulation of the activity of kinases such as PKC via binding to KRT1. Together with KRT1 and GNB2L1/RACK1, serves as a platform for SRC activation or inactivation. Plays a mechanistic adhesive role during telophase, required for the successful completion of cytokinesis. Integrin alpha-3/beta-1 provides a docking site for FAP (seprase) at invadopodia plasma membranes in a collagen-dependent manner and hence may participate in the adhesion, formation of invadopodia and matrix degradation processes, promoting cell invasion.

Isoform 5: Isoform 5 displaces isoform 1 in striated muscles. [UniProt]
Calculated MW 88 kDa
PTM The cysteine residues are involved in intrachain disulfide bonds.