ARG21154

anti-CD3 antibody [CT-3] (PE-Cyanine 5)

anti-CD3 antibody [CT-3] (PE-Cyanine 5) for Flow cytometry,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections and Chicken

Overview

Product Description PE-Cyanine 5-conjugated Mouse Monoclonal antibody [CT-3] recognizes CD3
Tested Reactivity Chk
Tested Application FACS, IHC-Fr, IHC-P
Specificity Chicken CD3. The clone CT-3 recognizes a complex of at least three polypeptides of Mr 20, 19, and 17 kDa (two of which are N-glycosylated) on chicken T cells. The antibody also coprecipitates a polypeptide of 90 kDa from digitonin solubilized T cell lysates, which can be reduced to two polypeptides of Mr 50 and 40 kDa.
Host Mouse
Clonality Monoclonal
Clone CT-3
Isotype IgG1, kappa
Target Name CD3
Antigen Species Chicken
Immunogen Chicken thymocytes and Ig- blood mononuclear cells
Conjugation PE-Cyanine 5
Full Name CD3d molecule, delta (CD3-TCR complex)
Alternate Names T-cell receptor T3 delta chain; T3D; IMD19; CD3-DELTA; CD antigen CD3d; T-cell surface glycoprotein CD3 delta chain

Application Instructions

Application Suggestion
Tested Application Dilution
FACS< 0.2 µg/10^6 cells
IHC-FrAssay-dependent
IHC-PAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 19 kDa

Properties

Form Liquid
Buffer PBS, Sodium azide and a stabilizing agent
Preservative Sodium azide
Concentration 0.1 mg/ml
Storage instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Symbol CD3D
Gene Full Name CD3d molecule, delta (CD3-TCR complex)
Background The protein encoded by this gene is part of the T-cell receptor/CD3 complex (TCR/CD3 complex) and is involved in T-cell development and signal transduction. The encoded membrane protein represents the delta subunit of the CD3 complex, and along with four other CD3 subunits, binds either TCR alpha/beta or TCR gamma/delta to form the TCR/CD3 complex on the surface of T-cells. Defects in this gene are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (SCIDBNK). Two transcript variants encoding different isoforms have been found for this gene. Other variants may also exist, but the full-length natures of their transcripts has yet to be defined. [provided by RefSeq, Feb 2009]
Function The CD3 complex mediates signal transduction. [UniProt]
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Images (1) Click the Picture to Zoom In

  • ARG21154 anti-CD3 antibody [CT-3] (PE-Cyanine 5) FACS image

    Flow Cytometry: Chicken peripheral blood lymphocytes stained with ARG21154 anti-CD3 antibody [CT-3] (PE-Cyanine 5) and ARG21162 anti-CD8a antibody [CT-8] (PE).