ARG65859

anti-CD3 antibody [SQab1713]

anti-CD3 antibody [SQab1713] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human

Cancer antibody; Developmental Biology antibody; Immune System antibody; Lymphocyte Marker antibody; Inflammatory Cell Marker antibody; T-cell Marker antibody; T-cell infiltration Study antibody; Tumor-infiltrating Lymphocyte Study antibody
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anti-CD3 antibody [SQab1713]

Recombinant Rabbit Monoclonal antibody [SQab1713] recognizes CD3

ARG65859 Arigo biolaboratories
5.0 out of 5 based on 1
anti-CD3 antibody [SQab1713] is rated 5.0 out of 5 by 1.

anti-CD3 antibody [SQab1713]

Overview

Product Description

Recombinant Rabbit Monoclonal antibody [SQab1713] recognizes CD3

Tested Reactivity Hu
Tested Application FACS, ICC/IF, IHC-P, IP, WB
Host Rabbit
Clonality Monoclonal
Clone SQab1713
Isotype IgG
Target Name CD3
Antigen Species Human
Immunogen Synthetic peptide around the N-terminus of Human CD3 epsilon.
Conjugation Un-conjugated
Full Name CD3e molecule, epsilon (CD3-TCR complex)
Alternate Names T-cell surface antigen T3/Leu-4 epsilon chain; T3E; TCRE; T-cell surface glycoprotein CD3 epsilon chain; IMD18; CD antigen CD3e

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:200 - 1:1000
ICC/IF1:50 - 1:2000
IHC-P1:100 - 1:200
IP1:15
WB1:1000 - 1:10000
Application Note IHC-P: Antigen Retrieval: Boil tissue section in Tris/EDTA buffer (pH 9.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 23 kDa

Properties

Form Liquid
Purification Purification with Protein A.
Buffer PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.
Preservative 0.01% Sodium azide
Stabilizer 40% Glycerol and 0.05% BSA
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 916 Human CD3E

Swiss-port # P07766 Human T-cell surface glycoprotein CD3 epsilon chain

Gene Symbol CD3E
Gene Full Name CD3e molecule, epsilon (CD3-TCR complex)
Background CD3 subunit complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins superfamily encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation.
Function CD3: Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed:2470098). In addition of this role of signal transduction in T-cell activation, CD3E plays an essential role in correct T-cell development. Initiates the TCR-CD3 complex assembly by forming the two heterodimers CD3D/CD3E and CD3G/CD3E. Participates also in internalization and cell surface down-regulation of TCR-CD3 complexes via endocytosis sequences present in CD3E cytosolic region (PubMed:10384095, PubMed:26507128). [UniProt]
Highlight Related Antibody Duos and Panels:
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Research Area Cancer antibody; Developmental Biology antibody; Immune System antibody; Lymphocyte Marker antibody; Inflammatory Cell Marker antibody; T-cell Marker antibody; T-cell infiltration Study antibody; Tumor-infiltrating Lymphocyte Study antibody

Images (6) Click the Picture to Zoom In

  • ARG65859 anti-CD3 antibody [SQab1713] WB image

    Western blot: 30 µg of Molt4 cell lysate stained with ARG65859 anti-CD3 antibody [SQab1713] at 1:500 dilution.

  • ARG65859 anti-CD3 antibody [SQab1713] ICC/IF image

    Immunofluorescence: Jurkat cells were fixed with 4% paraformaldehyde for 30 min at RT, permeabilized with 0.1% Triton X-100 for 10 min at RT then blocked with 10% Goat serum for half an hour at room temperature. Samples were stained with ARG65859 anti-CD3 antibody [SQab1713] (green) at 1:50 and 4°C. DAPI (blue) was used as the nuclear counter stain. Control: PBS and secondary antibody.

  • ARG65859 anti-CD3 antibody [SQab1713] FACS image

    Flow Cytometry: Jurkat cells were fixed with 4% paraformaldehyde for 10 min. The cells were then stained with ARG65859 anti-CD3 antibody [SQab1713] (blue) at 1:1000 dilution in 1x PBS/1% BSA for 30 min at room temperture, followed by Alexa Fluor® 488 labelled secondary antibody. Unlabelled sample (red) was used as a control.

  • ARG65859 anti-CD3 antibody [SQab1713] IHC-P image

    Immunohistochemistry: Formalin/PFA-fixed and paraffin-embedded sections of Human tonsil tissue stained with ARG65859 anti-CD3 antibody [SQab1713] at 1:200 dilution. Antigen Retrieval: Boil tissue section in Tris/EDTA buffer (pH 9.0).

  • ARG65859 anti-CD3 antibody [SQab1713] IP image

    Immunoprecipitation: 0.4 mg of Molt-4 whole cell lysate was immunoprecipitated (1:15 dilution) and stained with ARG65859 anti-CD3 antibody [SQab1713].

    Lane 1: Immunoprecipitation in Molt-4 whole cell lysate
    Lane 2: Rabbit IgG instead of Primary Ab in Molt-4 whole cell lysate
    Lane 3: Molt-4 whole cell lysate, 10 µg (input)

  • ARG65859 anti-CD3 antibody [SQab1713] IHC-P image

    Immunohistochemistry: Formalin/PFA-fixed and paraffin-embedded sections of Human colon tissue stained with ARG65859 anti-CD3 antibody [SQab1713] at 1:200 dilution. Antigen Retrieval: Boil tissue section in Tris/EDTA buffer (pH 9.0).

Customer's Feedback

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Review for anti-CD3 antibody [SQab1713]

Application:WB

Sample:Molt4

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:500

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC