ARG22157

anti-CD3 antibody [UCHT1] (APC-Cyanine 7)

anti-CD3 antibody [UCHT1] (APC-Cyanine 7) for Blocking,Cellular activation ,Flow cytometry,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Overview

Product Description APC-Cyanine 7-conjugated Mouse Monoclonal antibody [UCHT1] recognizes CD3
Tested Reactivity Hu
Tested Application BL, Cell-Act , FACS, ICC/IF, IHC-Fr, IHC-P, WB
Specificity Human CD3
Host Mouse
Clonality Monoclonal
Clone UCHT1
Isotype IgG1, kappa
Target Name CD3
Antigen Species Human
Immunogen Human peripheral blood lymphocytes from a patient with Sézary's syndrome and infant thymocytes
Conjugation APC-Cyanine 7
Alternate Names T-cell receptor T3 delta chain; T3D; IMD19; CD3-DELTA; CD antigen CD3d; T-cell surface glycoprotein CD3 delta chain

Application Instructions

Application Suggestion
Tested Application Dilution
BLAssay-dependent
Cell-Act Assay-dependent
FACS10 µl/10^6 cells
ICC/IFAssay-dependent
IHC-FrAssay-dependent
IHC-PAssay-dependent
WBAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Buffer PBS, 0.1% Sodium azide and Sucrose.
Preservative 0.1% Sodium azide
Stabilizer Sucrose
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 915 Human CD3D

Swiss-port # P04234 Human T-cell surface glycoprotein CD3 delta chain

Gene Symbol CD3D
Gene Full Name CD3d molecule, delta (CD3-TCR complex)
Background The protein encoded by this gene is part of the T-cell receptor/CD3 complex (TCR/CD3 complex) and is involved in T-cell development and signal transduction. The encoded membrane protein represents the delta subunit of the CD3 complex, and along with four other CD3 subunits, binds either TCR alpha/beta or TCR gamma/delta to form the TCR/CD3 complex on the surface of T-cells. Defects in this gene are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (SCIDBNK). Two transcript variants encoding different isoforms have been found for this gene. Other variants may also exist, but the full-length natures of their transcripts has yet to be defined. [provided by RefSeq, Feb 2009]
Function The CD3 complex mediates signal transduction. [UniProt]
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Calculated MW 19 kDa

Images (2) Click the Picture to Zoom In

  • ARG22157 anti-CD3 antibody [UCHT1] (APC-Cyanine 7) FACS image

    Flow Cytometry: Human peripheral blood lymphocytes stained with ARG22157 anti-CD3 antibody [UCHT1] (APC-Cyanine 7) and ARG21258 anti-CD19 antibody [SJ25-C1] (FITC).

  • ARG22157 anti-CD3 antibody [UCHT1] (APC-Cyanine 7) FACS image

    Flow Cytometry: Human peripheral blood lymphocytes stained with ARG22157 anti-CD3 antibody [UCHT1] (APC-Cyanine 7).