anti-CD3 antibody [UCHT1] (FITC)

anti-CD3 antibody [UCHT1] (FITC) for Blocking,Cellular activation ,Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,Western blot and Human

Cancer antibody; Developmental Biology antibody; Immune System antibody


Product Description FITC-conjugated Mouse Monoclonal antibody [UCHT1] recognizes CD3
Tested Reactivity Hu
Tested Application BL, Cell-Act , FACS, ICC/IF, IHC-Fr, IHC-P, WB
Host Mouse
Clonality Monoclonal
Clone UCHT1
Isotype IgG1, kappa
Target Name CD3
Antigen Species Human
Immunogen Human peripheral blood lymphocytes from a patient with Sézary's syndrome and infant thymocytes
Conjugation FITC
Alternate Names T-cell receptor T3 delta chain; T3D; IMD19; CD3-DELTA; CD antigen CD3d; T-cell surface glycoprotein CD3 delta chain

Application Instructions

Application Suggestion
Tested Application Dilution
Cell-Act Assay-dependent
FACS10 µl/10^6 cells
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.


Form Liquid
Buffer PBS and 0.1% Sodium azide.
Preservative 0.1% Sodium azide
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 915 Human CD3D

Swiss-port # P04234 Human T-cell surface glycoprotein CD3 delta chain

Gene Symbol CD3D
Gene Full Name CD3d molecule, delta (CD3-TCR complex)
Background CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation.
The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.
Function The CD3 complex mediates signal transduction. [UniProt]
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Research Area Cancer antibody; Developmental Biology antibody; Immune System antibody
Calculated MW 19 kDa

Images (1) Click the Picture to Zoom In

  • ARG62806 anti-CD3 antibody [UCHT1] (FITC) FACS image

    Flow Cytometry: Human peripheral blood lymphocytes stained with ARG23891 anti-CD23 antibody [Tü1] (PE) and ARG62806 anti-CD3 antibody [UCHT1] (FITC).

Clone References

Different composition of the human and the mouse gammadelta T cell receptor explains different phenotypes of CD3gamma and CD3delta immunodeficiencies.

Siegers GM et al.
J Exp Med.,  (2007)




Expansion of regulatory T cells in patients with Langerhans cell histiocytosis.

Senechal B et al.
PLoS Med.,  (2007)




Inherited and somatic CD3zeta mutations in a patient with T-cell deficiency.

Rieux-Laucat F et al.
N Engl J Med.,  (2006)




Identification and characterization of human pulmonary dendritic cells.

Demedts IK et al.
Am J Respir Cell Mol Biol.,  (2005)