ARG53820

anti-CD3 antibody [UCHT1] (PE)

anti-CD3 antibody [UCHT1] (PE) for Flow cytometry and Human,Primates

Cancer antibody; Developmental Biology antibody; Immune System antibody

Overview

Product Description PE-conjugated Mouse Monoclonal antibody [UCHT1] recognizes CD3
Tested Reactivity Hu, NHuPrm
Tested Application FACS
Specificity The clone UCHT1 recognizes the CD3 antigen of the TCR/CD3 complex on mature human T cells. The UCHT1 antibody reacts with the epsilon chain of the CD3 complex.
HLDA I; WS Code T 3
HLDA III; WS Code T 126
HLDA III; WS Code T 471
HLDA VI; WS Code T 6T-CD3.1
Host Mouse
Clonality Monoclonal
Clone UCHT1
Isotype IgG1
Target Name CD3
Antigen Species Human
Immunogen human thymocytes followed by Sezary T cells
Conjugation PE
Alternate Names T-cell receptor T3 delta chain; T3D; IMD19; CD3-DELTA; CD antigen CD3d; T-cell surface glycoprotein CD3 delta chain

Application Instructions

Application Suggestion
Tested Application Dilution
FACS20 µl / 10^6 cells
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Note The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
Buffer PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA
Preservative 15 mM Sodium azide
Stabilizer 0.2% (w/v) high-grade protease free BSA
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 915 Human CD3D

Swiss-port # P04234 Human T-cell surface glycoprotein CD3 delta chain

Gene Symbol CD3D
Gene Full Name CD3d molecule, delta (CD3-TCR complex)
Background CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation.
The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.
Function The CD3 complex mediates signal transduction. [UniProt]
Highlight Related products:
CD3 antibodies; CD3 ELISA Kits; CD3 Duos / Panels; CD3 recombinant proteins; Anti-Mouse IgG secondary antibodies;
Related news:
New antibody panels and duos for Tumor immune microenvironment
Tumor-Infiltrating Lymphocytes (TILs)
Exploring Antiviral Immune Response
Research Area Cancer antibody; Developmental Biology antibody; Immune System antibody
Calculated MW 19 kDa

Images (2) Click the Picture to Zoom In

  • ARG53820 anti-CD3 antibody [UCHT1] (PE) FACS image

    Flow Cytometry: Human peripheral whole blood stained with ARG53820 anti-CD3 antibody [UCHT1] (PE) (20 µl reagent / 100 µl of peripheral whole blood).

  • ARG53820 anti-CD3 antibody [UCHT1] (PE) FACS image

    Flow Cytometry: Separation of human CD3 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed). Human peripheral whole blood stained with ARG53820 anti-CD3 antibody [UCHT1] (PE) (20 µl reagent / 100 µl of peripheral whole blood).

Clone References

Different composition of the human and the mouse gammadelta T cell receptor explains different phenotypes of CD3gamma and CD3delta immunodeficiencies.

Siegers GM et al.
J Exp Med.,  (2007)

publication_link

 

hr_line

Expansion of regulatory T cells in patients with Langerhans cell histiocytosis.

Senechal B et al.
PLoS Med.,  (2007)

publication_link

 

hr_line

Inherited and somatic CD3zeta mutations in a patient with T-cell deficiency.

Rieux-Laucat F et al.
N Engl J Med.,  (2006)

publication_link

 

hr_line

Identification and characterization of human pulmonary dendritic cells.

Demedts IK et al.
Am J Respir Cell Mol Biol.,  (2005)

publication_link

 

hr_line

Crystal structure of a human CD3-epsilon/delta dimer in complex with a UCHT1 single-chain antibody fragment.

Arnett KL et al.
Proc Natl Acad Sci U S A.,  (2004)

publication_link

 

hr_line

TCR dynamics in human mature T lymphocytes lacking CD3 gamma.

Torres PS et al.
J Immunol.,  (2003)

publication_link

 

hr_line