ARG10870

anti-CD51 / Integrin alpha V antibody [P2W7]

anti-CD51 / Integrin alpha V antibody [P2W7] for Flow cytometry,Immunohistochemistry,ICC/IF,Immunoprecipitation,Western blot and Human

Overview

Product Description Mouse Monoclonal antibody [P2W7] recognizes CD51 / Integrin alpha V
Tested Reactivity Hu
Tested Application FACS, ICC/IF, IHC, IP, WB
Host Mouse
Clonality Monoclonal
Clone P2W7
Target Name CD51 / Integrin alpha V
Antigen Species Human
Immunogen Ocular melanoma cell line (V+B2), a clone expressing high levels of alpha-v, beta-1
Conjugation Un-conjugated
Alternate Names CD51; VNRA; CD antigen CD51; VTNR; Vitronectin receptor subunit alpha; Integrin alpha-V; MSK8

Application Instructions

Application Suggestion
Tested Application Dilution
FACSAssay-dependent
ICC/IFAssay-dependent
IHCAssay-dependent
IPAssay-dependent
WBAssay-dependent
Application Note WB: Human alpha v is about 150-160 kDa under non-reducing conditions. Under reducing conditions it is cleaved into 2 bands of about 125-130 kDa + 20-25 kDa.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified by affinity chromatography.
Buffer PBS and 0.02% Sodium azide.
Preservative 0.02% Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 3685 Human ITGAV

Swiss-port # P06756 Human Integrin alpha-V

Gene Symbol ITGAV
Gene Full Name integrin, alpha V
Background This gene encodes a protein that is a member of the integrin superfamily. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This protein undergoes post-translational cleavage to yield disulfide-linked heavy and light chains that combine with multiple integrin beta chains to form different integrins. This protein has been shown to heterodimerize with beta 1, beta 3, beta 5, beta 6, and beta 8; the heterodimer of alpha v and beta 3 is the Vitronectin receptor. This protein interacts with several extracellular matrix proteins to mediate cell adhesion and may play a role in cell migration. It is proposed that this protein may regulate angiogenesis and cancer progression. Alternative splicing results in multiple transcript variants that encode different protein isoforms. Note that the integrin alpha 5 and integrin alpha V chains are produced by distinct genes. [provided by RefSeq, Jan 2015]
Function The alpha-V (ITGAV) integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin and vWF. They recognize the sequence R-G-D in a wide array of ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. [UniProt]
Calculated MW 116 kDa