ARG20960

anti-CD62E antibody [1.2B6] (APC)

anti-CD62E antibody [1.2B6] (APC) for Flow cytometry,IHC-Frozen sections,ICC/IF,Western blot,ELISA,Blocking and Human,Pig

Overview

Product Description APC-conjugated Mouse Monoclonal antibody [1.2B6] recognizes CD62E
Tested Reactivity Hu, Pig
Tested Application BL, ELISA, FACS, ICC/IF, IHC-Fr, WB
Specificity Human CD62E & CD62P/Porcine CD62E. The clone 1.2B6 recognizes a common epitope shared by CD62E and CD62P.
Host Mouse
Clonality Monoclonal
Clone 1.2B6
Isotype IgG1, kappa
Target Name CD62E
Species Human
Immunogen TNF stimulated human endothelial cells
Conjugation APC
Full Name selectin E
Alternate Names E-selectin; CD62E; ELAM-1; ELAM1; CD antigen CD62E; ELAM; Endothelial leukocyte adhesion molecule 1; Leukocyte-endothelial cell adhesion molecule 2; ESEL; LECAM2; CD62 antigen-like family member E

Application Instructions

Application Suggestion
Tested Application Dilution
BLAssay-dependent
ELISAAssay-dependent
FACS10 µl/10^6 cells
ICC/IFAssay-dependent
IHC-FrAssay-dependent
WBAssay-dependent
Application Note WB: Under non-reducing condition.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 67 kDa

Properties

Form Liquid
Buffer PBS, Sodium azide and a stabilizing agent
Preservative Sodium azide
Storage instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 397508 Pig SELE

GeneID: 6401 Human SELE

Swiss-port # P16581 Human E-selectin

Swiss-port # P98110 Pig E-selectin

Gene Symbol SELE
Gene Full Name selectin E
Background The protein encoded by this gene is found in cytokine-stimulated endothelial cells and is thought to be responsible for the accumulation of blood leukocytes at sites of inflammation by mediating the adhesion of cells to the vascular lining. It exhibits structural features such as the presence of lectin- and EGF-like domains followed by short consensus repeat (SCR) domains that contain 6 conserved cysteine residues. These proteins are part of the selectin family of cell adhesion molecules. Adhesion molecules participate in the interaction between leukocytes and the endothelium and appear to be involved in the pathogenesis of atherosclerosis. [provided by RefSeq, Jul 2008]
Function Cell-surface glycoprotein having a role in immunoadhesion. Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with PSGL1/SELPLG. May have a role in capillary morphogenesis. [UniProt]