ARG10601

anti-CYP19A1 / Aromatase antibody

anti-CYP19A1 / Aromatase antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes CYP19A1 / Aromatase
Tested Reactivity Rat
Tested Application IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name CYP19A1 / Aromatase
Antigen Species Rat
Immunogen Synthetic peptide around the C-terminus of Rat Aromatase. (LKRFHVKTLQKRCIENMPKNND)
Conjugation Un-conjugated
Full Name cytochrome P450, family 19, subfamily A, polypeptide 1
Alternate Names ARO1; CPV1; CYAR; Aromatase; Cytochrome P450 19A1; CYP19; Cytochrome P-450AROM; P-450AROM; Estrogen synthase; EC 1.14.14.14; CYPXIX; ARO

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P0.5 - 1 µg/ml
WB0.5 - 1 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW ~ 58 kDa

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS, 0.025% Sodium azide and 2.5% BSA
Preservative 0.025% Sodium azide
Stabilizer 2.5% BSA
Concentration 0.5 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Symbol Cyp19a1
Gene Full Name cytochrome P450, family 19, subfamily A, polypeptide 1
Background This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and catalyzes the last steps of estrogen biosynthesis. Mutations in this gene can result in either increased or decreased aromatase activity; the associated phenotypes suggest that estrogen functions both as a sex steroid hormone and in growth or differentiation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]
Function Catalyzes the formation of aromatic C18 estrogens from C19 androgens. [UniProt]
Cellular Localization Membrane; Peripheral membrane protein
PTM Phosphorylated in vitro by PKA and PKG/PRKG1. These phosphorylations inhibit the catalytic activity as measured by estrone synthesis from androstenedione (36% decrease for PKA and 30% for PKG/PRKG1).

Images (2) Click the Picture to Zoom In

  • ARG10601 anti-CYP19A1 / Aromatase antibody IHC-P image

    Immunohistochemistry: Rat brain tissue stained with ARG10601 anti-CYP19A1 / Aromatase antibody.

  • ARG10601 anti-CYP19A1 / Aromatase antibody WB image

    Western blot: 1) Rat brain, 2) Rat thymus, and 3) Rat kidney tissue lysates stained with ARG10601 anti-CYP19A1 / Aromatase antibody.