ARG10509

anti-Calcitonin antibody [16B5]

anti-Calcitonin antibody [16B5] for ELISA,Flow cytometry,Western blot and Human

Cancer antibody; Cell Biology and Cellular Response antibody; Neuroscience antibody; Signaling Transduction antibody

Overview

Product Description Mouse Monoclonal antibody [16B5] recognizes Calcitonin
Tested Reactivity Hu
Tested Application ELISA, FACS, WB
Specificity Human calcitonin and procalcitonin
Host Mouse
Clonality Monoclonal
Clone 16B5
Isotype IgG2b
Target Name Calcitonin
Antigen Species Human
Immunogen Balb/c mice immunized with calcitonin conjugated with carrier protein.
Epitope aa. 72-81 of PCT.
Conjugation Un-conjugated
Alternate Names KC; CGRP-I; Calcitonin gene-related peptide 1; CGRP1; CALC1; Calcitonin gene-related peptide I; Alpha-type CGRP; CGRP; CT

Application Instructions

Application Note Sandwich ELISA: Recommended antibody pairs in sandwich immunoassay for procalcitonin (capture - detection): ARG10509 - ARG10152
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Protein A affinity purified.
Buffer PBS (pH.2)) and 0.1 % Sodium azide
Preservative 0.1 % Sodium azide
Concentration 1.0-2.0 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 796 Human CALCA

Swiss-port # P06881 Human Calcitonin gene-related peptide 1

Gene Symbol CALCA
Gene Full Name calcitonin-related polypeptide alpha
Background This gene encodes the peptide hormones calcitonin, calcitonin gene-related peptide and katacalcin by tissue-specific alternative RNA splicing of the gene transcripts and cleavage of inactive precursor proteins. Calcitonin is involved in calcium regulation and acts to regulate phosphorus metabolism. Calcitonin gene-related peptide functions as a vasodilator and as an antimicrobial peptide while katacalcin is a calcium-lowering peptide. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Aug 2014]
Function CGRP induces vasodilation. It dilates a variety of vessels including the coronary, cerebral and systemic vasculature. Its abundance in the CNS also points toward a neurotransmitter or neuromodulator role. It also elevates platelet cAMP. [UniProt]
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Neuroscience antibody; Signaling Transduction antibody

Images (2) Click the Picture to Zoom In

  • ARG10509 anti-Calcitonin antibody [16B5] sELISA image

    Sandwich ELISA: Calibration curves for MAb combinations clone 16B5 - 42. The immunoassay was pre-coated with 1 μg/well of ARG10152 anti-Procalcitonin antibody [42] as capture antibodies. Recombinant human proalcitonin was added to the wells and incubated for 30 min at RT. After wash away unbonded reagents. A Eu3+-labeled detection antibody, ARG10509 anti-Calcitonin antibody [16B5] (0.1 μg/wel) was added and incubated.

  • ARG10509 anti-Calcitonin antibody [16B5] Immuongen location image

    The epitope of ARG10509 anti-Calcitonin antibody [16B5] antibody is located at a.a. 72-81 of Proalcitonin.