ARG54735

anti-Calpain 1 antibody

anti-Calpain 1 antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Cell Biology and Cellular Response antibody; Signaling Transduction antibody

Overview

Product Description Mouse Monoclonal antibody recognizes Calpain 1
Tested Reactivity Hu
Tested Application FACS, IHC-P, WB
Host Mouse
Clonality Monoclonal
Clone 1376CT809.24.85
Target Name Calpain 1
Antigen Species Human
Immunogen KLH-conjugated synthetic peptide from Human CAPN1 protein (NP_001185797.1).
Conjugation Un-conjugated
Alternate Names EC 3.4.22.52; CANP1; Calpain-1 large subunit; Calpain-1 catalytic subunit; Calpain mu-type; muCANP; Cell proliferation-inducing gene 30 protein; Micromolar-calpain; CANP 1; CANP; muCL; CANPL1; Calcium-activated neutral proteinase 1

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:25
IHC-PAssay-dependent
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Purification Protein G purified
Buffer PBS and 0.09% (W/V) Sodium azide
Preservative 0.09% (W/V) Sodium azide
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 823 Human CAPN1

Swiss-port # P07384 Human Calpain-1 catalytic subunit

Gene Symbol CAPN1
Gene Full Name calpain 1, (mu/I) large subunit
Background The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 1. Several transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Nov 2010]
Function Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. [From Uniprot]
Cellular Localization Cytoplasm. Cell membrane. Note=Translocates to the plasma membrane upon Ca(2+) binding
Highlight Related products:
Calpain1 antibodies;
Related poster download:
The NF-kappa B Pathways.pdf
Research Area Cell Biology and Cellular Response antibody; Signaling Transduction antibody
Calculated MW 82 kDa
PTM Undergoes calcium-induced successive autoproteolytic cleavages that generate a membrane-bound 78 kDa active form and an intracellular 75 kDa active form. Calpastatin reduces with high efficiency the transition from 78 kDa to 75 kDa calpain forms.

Images (3) Click the Picture to Zoom In

  • ARG54735 anti-Calpain 1 antibody WB image

    Western blot: 30 μg of MCF-7, HeLa, U87-MG and HCT116 cell lysates stained with ARG54735 anti-Calpain 1 antibody at 1:500 dilution.

  • ARG54735 anti-Calpain 1 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human kidney section stained with ARG54735 anti-Calpain 1 antibody at 1:25 dilution.

  • ARG54735 anti-Calpain 1 antibody FACS image

    Flow Cytometry: HeLa cells stained with ARG54735 anti-Calpain 1 antibody (right histogram) at 1:25 dilution or isotype control antibody (left histogram), followed by incubation with Alexa Fluor® 488 labelled secondary antibody.