ARG10706

anti-Cas9 antibody [3F9]

anti-Cas9 antibody [3F9] for Western blot,ICC/IF,IHC-Frozen sections and Bacteria

Overview

Product Description Mouse Monoclonal antibody [3F9] recognizes Cas9
Tested Reactivity Bacteria
Tested Application ICC/IF, IHC-Fr, WB
Host Mouse
Clonality Monoclonal
Clone 3F9
Isotype IgG
Target Name Cas9
Antigen Species Bacteria
Immunogen N-terminal region, aa. 1-608 of Cas9 sequence CDJ55032.1 from S. pyogenes, expressed in and purified from E. coli.
Conjugation Un-conjugated

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:1000 - 1:2000
IHC-FrAssay-dependent
WB1:1000 - 1: 20000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification.
Buffer PBS, 5 mM Sodium azide and 50% Glycerol.
Preservative 5 mM Sodium azide
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Highlight Related products:
Cas9 antibodies; Anti-Mouse IgG secondary antibodies;
Related news:
How snakes lose the limbs? CRISPR/Cas9 technique tell you the answer

Images (2) Click the Picture to Zoom In

  • ARG10706 anti-Cas9 antibody [3F9] ICC/IF image

    Immunocytochemistry: Transfected Hek293 cells overexpressing the N-terminal 1-608 amino acids of S. pyogenes  Cas9. The cells were stained with ARG10706 anti-Cas9 antibody [3F9] (red), and these cells also co-stained with ARG10734 anti-Cas9 antibody to the same construct in green, giving a yellow color. The N-terminal construct contains a nuclear localization sequence and so is predominantly nuclear in localization. Most Hek293 cells in this field are not transfected so only the nuclei of these cells can be visualized with the blue DAPI DNA stain.

  • ARG10706 anti-Cas9 antibody [3F9] WB image

    Western blot: 1) MW marker, 2) crude protein extract from Hek293 culture transfected with the immunogen, the N-terminal 1-608 amino acids, 3) Non-transfected control Hek293 cell extract, and 4) 40 ng of Streptococcus pyogenes  Cas9 (full length) was stained with ARG10706 anti-Cas9 antibody [3F9] at 1:1000 dilution.