ARG10705

anti-Cas9 antibody [6F7]

anti-Cas9 antibody [6F7] for ICC/IF,IHC-Frozen sections,Western blot and Bacteria

Overview

Product Description

Mouse Monoclonal antibody [6F7] recognizes Cas9

Tested Reactivity Bacteria
Tested Application ICC/IF, IHC-Fr, WB
Host Mouse
Clonality Monoclonal
Clone 6F7
Isotype IgG1
Target Name Cas9
Antigen Species Bacteria
Immunogen C-terminal region of S. aureus Cas9, aa. 803-1053 of sequence CCK74173, expressed in and purified from E. coli.
Conjugation Un-conjugated

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:1000 - 1:5000
IHC-Fr1:1000 - 1:5000
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification.
Buffer PBS and 50% Glycerol.
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Highlight Related products:
Cas9 antibodies; Anti-Mouse IgG secondary antibodies;
Related news:
How snakes lose the limbs? CRISPR/Cas9 technique tell you the answer

Images (2) Click the Picture to Zoom In

  • ARG10705 anti-Cas9 antibody [6F7] ICC/IF image

    Immunocytochemistry: Transfected HEK293 cells with GFP-Cas9 (SA) fusion protein were stained with ARG10705 anti-Cas9 antibody [6F7] (red) at 1:1000 dilution. GFP is expressed in the transfected cells (green). Only transfected cells are reactive with ARG10705 anti-Cas9 antibody [6F7] antibody, which appear in a yellow-orange color (merge). Nuclei are visualized in blue with Hoechst staining.

  • ARG10705 anti-Cas9 antibody [6F7] WB image

    Western blot: 1) HEK293 cells which overexpressed fusion protein containing GFP and c-terminus of Cas9 from S. aureus, and 2) Non-transfected HEK293 cells stained with ARG10705 anti-Cas9 antibody [6F7] at 1: 1000 dilution.