anti-Cas9 antibody [6F7]
anti-Cas9 antibody [6F7] for ICC/IF,IHC-Frozen sections,Western blot and Bacteria
Mouse Monoclonal antibody [6F7] recognizes Cas9
|Tested Application||ICC/IF, IHC-Fr, WB|
|Immunogen||C-terminal region of S. aureus Cas9, aa. 803-1053 of sequence CCK74173, expressed in and purified from E. coli.|
|Application Note||* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.|
|Buffer||PBS and 50% Glycerol.|
|Storage instruction||For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.|
|Note||For laboratory research only, not for drug, diagnostic or other use.|
Cas9 antibodies; Anti-Mouse IgG secondary antibodies;
How snakes lose the limbs? CRISPR/Cas9 technique tell you the answer
Images (2) Click the Picture to Zoom In
ARG10705 anti-Cas9 antibody [6F7] ICC/IF image
Immunocytochemistry: Transfected HEK293 cells with GFP-Cas9 (SA) fusion protein were stained with ARG10705 anti-Cas9 antibody [6F7] (red) at 1:1000 dilution. GFP is expressed in the transfected cells (green). Only transfected cells are reactive with ARG10705 anti-Cas9 antibody [6F7] antibody, which appear in a yellow-orange color (merge). Nuclei are visualized in blue with Hoechst staining.
ARG10705 anti-Cas9 antibody [6F7] WB image
Western blot: 1) HEK293 cells which overexpressed fusion protein containing GFP and c-terminus of Cas9 from S. aureus, and 2) Non-transfected HEK293 cells stained with ARG10705 anti-Cas9 antibody [6F7] at 1: 1000 dilution.