anti-Cas9 antibody for Western blot,ICC/IF,IHC-Frozen sections and Bacteria
|Product Description||Chicken Polyclonal antibody recognizes Cas9|
|Tested Application||ICC/IF, IHC-Fr, WB|
|Immunogen||C-terminal region of S. aureus Cas9, aa. 803-1053 of sequence CCK74173, expressed in and purified from E. coli.|
|Application Note||* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.|
|Buffer||PBS and 0.02% Sodium azide.|
|Preservative||0.02% Sodium azide|
|Storage instruction||For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.|
|Note||For laboratory research only, not for drug, diagnostic or other use.|
Cas9 antibodies; Anti-Chicken IgY secondary antibodies;
How snakes lose the limbs? CRISPR/Cas9 technique tell you the answer
Images (2) Click the Picture to Zoom In
ARG10681 anti-Cas9 antibody ICC/IF image
Immunocytochemistry: Transfected HEK293 cells which overexpressing GFP-Cas9 (SA) fusion protein were stained with ARG10681 anti-Cas9 antibody. Cells which are transfected with GFP-Cas9 are bright green (left panel). Staining with ARG10681 anti-Cas9 antibody is shown in red in middle panel. In merged image (right panel), most Hek293 cells are not transfected so only the nucleus of these cells can be visualized with a blue DNA stain. Red antibody staining is only seen in cells which express GFP, as expected, and the superimposition of green and red results in an orange signal.
ARG10681 anti-Cas9 antibody WB image
Western blot: 1) HEK293 cells overexpressing a fusion protein containing GFP and the C-terminus of Cas9 from S. aureus, and 2) Non-transfected HEK293 cells stained with ARG10681 anti-Cas9 antibody at 1:1000 dilution.