ARG10681

anti-Cas9 antibody

anti-Cas9 antibody for Western blot,ICC/IF,IHC-Frozen sections and Bacteria

Overview

Product Description Chicken Polyclonal antibody recognizes Cas9
Tested Reactivity Bacteria
Tested Application ICC/IF, IHC-Fr, WB
Host Chicken
Clonality Polyclonal
Isotype IgY
Target Name Cas9
Antigen Species Bacteria
Immunogen C-terminal region of S. aureus Cas9, aa. 803-1053 of sequence CCK74173, expressed in and purified from E. coli.
Conjugation Un-conjugated

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:1000 - 1:5000
IHC-Fr1:1000 - 1:5000
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Buffer PBS and 0.02% Sodium azide.
Preservative 0.02% Sodium azide
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Highlight Related products:
Cas9 antibodies; Anti-Chicken IgY secondary antibodies;
Related news:
How snakes lose the limbs? CRISPR/Cas9 technique tell you the answer

Images (2) Click the Picture to Zoom In

  • ARG10681 anti-Cas9 antibody ICC/IF image

    Immunocytochemistry: Transfected HEK293 cells which overexpressing GFP-Cas9 (SA) fusion protein were stained with ARG10681 anti-Cas9 antibody. Cells which are transfected with GFP-Cas9 are bright green (left panel). Staining with ARG10681 anti-Cas9 antibody is shown in red in middle panel. In merged image (right panel), most Hek293 cells are not transfected so only the nucleus of these cells can be visualized with a blue DNA stain. Red antibody staining is only seen in cells which express GFP, as expected, and the superimposition of green and red results in an orange signal.

  • ARG10681 anti-Cas9 antibody WB image

    Western blot: 1) HEK293 cells overexpressing a fusion protein containing GFP and the C-terminus of Cas9 from S. aureus, and 2) Non-transfected HEK293 cells stained with ARG10681 anti-Cas9 antibody at 1:1000 dilution.