ARG10734

anti-Cas9 antibody

anti-Cas9 antibody for ICC/IF,IHC-Frozen sections,Western blot and Bacteria

Overview

Product Description Rabbit Polyclonal antibody recognizes Cas9
Tested Reactivity Bacteria
Tested Application ICC/IF, IHC-Fr, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Cas9
Antigen Species Bacteria
Immunogen N-terminal region, aa. 1-608 and C-terminal region, aa. 814-1372 of Cas9 sequence CDJ55032.1 from S. pyogenes, expressed in and purified from E. coli.
Conjugation Un-conjugated

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:1000 - 1:2000
IHC-FrAssay-dependent
WB1:1000 - 1:10000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification.
Buffer PBS and 50% Glycerol.
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Highlight Related products:
Cas9 antibodies; Anti-Rabbit IgG secondary antibodies;
Related news:
How snakes lose the limbs? CRISPR/Cas9 technique tell you the answer

Images (3) Click the Picture to Zoom In

  • ARG10734 anti-Cas9 antibody ICC/IF image

    Immunocytochemistry: Hek293 cell culture transfected with a construct including the C-terminal 814-1372 amino acids of Staphylococcus  pyogenes Cas9. The cells were stained with ARG10734 anti-Cas9 antibody (red) and also with a chicken antibody to S. pyogenes Cas9 (green). A cell expressing Cas9 therefore appears yellow. Most Hek293 cells are not transfected so only the nucleus of these cells can be visualized with the blue DNA stain.

  • ARG10734 anti-Cas9 antibody WB image

    Western blot: 1) 100 ng, and 2) 10 ng of full length Cas9 protein from Streptococcus pyogenes was stained with ARG10734 anti-Cas9 antibody at 1:2000 dilution. This antibody recognizes full length Cas9 protein at 160 kDa and a proteolytic fragment at 130 kDa.

  • ARG10734 anti-Cas9 antibody WB image

    Western blot: 1) HEK293 (non-transfected), 2) transfected HEK293 expressing the C-terminal aa. 814-1372 of Cas9 from S. pyogenes, 3) transfected HEK293 expressing the N-terminal 608 amino acids of Cas9 from S. pyogenes and 4) 40 ng of full length S. pyogenes Cas9 protein. The blots were stained with ARG10734 anti-Cas9 antibody (green) at 1: 1000 dilution.