ARG52893

anti-Connexin 43 antibody

anti-Connexin 43 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Cell Biology and Cellular Response antibody; Signaling Transduction antibody; Cardiomyocyte Cell Surface Marker antibody
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Overview

Product Description Rabbit Polyclonal antibody recognizes Connexin 43
Tested Reactivity Hu, Ms, Rat
Tested Application IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Connexin 43
Antigen Species Rat
Immunogen Synthetic peptide derived from a segment of the 3rd cytoplasmic domain of rat connexin-43.
Conjugation Un-conjugated
Alternate Names Gap junction 43 kDa heart protein; CX43; PPKCA; CMDR; Gap junction alpha-1 protein; HSS; AVSD3; Connexin-43; HLHS1; EKVP; GJAL; ODDD; Cx43

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:100
WB1:25
Application Note IHC-P: Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
Incubation Time: 10 min at RT.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Heart, MCF-7

Properties

Form Liquid
Purification Immunogen affinity purified
Buffer PBS (pH 7.6), 1% BSA and < 0.1% Sodium azide
Preservative < 0.1% Sodium azide
Stabilizer 1% BSA
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 14609 Mouse GJA1

GeneID: 24392 Rat GJA1

GeneID: 2697 Human GJA1

Gene Symbol Gja1
Gene Full Name gap junction protein, alpha 1
Background The connexins are a group of gap junction proteins which form a hexamer to compose a connexon. Clusters of connexons form a gap junction through which low molecular weight proteins may diffuse from cell to cell. Connexins are also thought to be essential for the development of labor contractions.
Function Gap junction protein that acts as a regulator of bladder capacity. A gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. Negative regulator of bladder functional capacity: acts by enhancing intercellular electrical and chemical transmission, thus sensitizing bladder muscles to cholinergic neural stimuli and causing them to contract (By similarity). [UniProt]
Cellular Localization Cytoplasm
Highlight Related Antibody Duos and Panels:
ARG30142 Cardiomyocyte Cell Surface Marker Antibody Panel (Cx43, Desmin, SIRP)
Related products:
Connexin43 antibodies; Connexin43 Duos / Panels; Anti-Rabbit IgG secondary antibodies;
Related news:
Molecular mechanisms of labor initiation found
Research Area Cell Biology and Cellular Response antibody; Signaling Transduction antibody; Cardiomyocyte Cell Surface Marker antibody
Calculated MW 43 kDa
PTM Phosphorylated at Ser-368 by PRKCG; phosphorylation induces disassembly of gap junction plaques and inhibition of gap junction activity (By similarity). Phosphorylation at Ser-325, Ser-328 and Ser-330 by CK1 modulates gap junction assembly. Phosphorylation at Ser-368 by PRKCD triggers its internalization into small vesicles leading to proteasome-mediated degradation (By similarity).
Sumoylated with SUMO1, SUMO2 and SUMO3, which may regulate the level of functional Cx43 gap junctions at the plasma membrane. May be desumoylated by SENP1 or SENP2.
S-nitrosylation at Cys-271 is enriched at the muscle endothelial gap junction in arteries, it augments channel permeability and may regulate of smooth muscle cell to endothelial cell communication.

Images (2) Click the Picture to Zoom In

  • ARG52893 anti-Connexin 43 antibody WB image

    Western blot: 20 µg of MCF7 and Mouse brain lysates stained with ARG52893 anti-Connexin 43 antibody at 1:30 dilution.

  • ARG52893 anti-Connexin 43 antibody IHC-P image

    Immunohistochemistry: Human Heart stained with ARG52893 anti-Connexin 43 antibody.

Customer's Feedback

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Review for anti-Connexin 43 antibody

Application:WB

Sample:MCF7 and Mouse brain

Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:1:30

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC