ARG54342

anti-DFF40 / CAD antibody

anti-DFF40 / CAD antibody for ICC/IF,Western blot and Mouse

Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Gene Regulation antibody; Metabolism antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes DFF40 / CAD
Tested Reactivity Ms
Tested Application ICC/IF, WB
Specificity This antibody recognizes human, mouse, and rat DFF40 / CAD (40 kDa).
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name DFF40 / CAD
Antigen Species Mouse
Immunogen Peptide corresponding to aa. 147-164 near the center of murine DFF40 / CAD (accession no. NP_031885). The sequence differs from human DFF40 / CAD by two amino acids.
Conjugation Un-conjugated
Alternate Names Caspase-activated DNase; DFF40; DFF-40; Caspase-activated deoxyribonuclease; DNA fragmentation factor 40 kDa subunit; Caspase-activated nuclease; CPAN; DNA fragmentation factor subunit beta; EC 3.-.-.-; DFF2; CAD

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IFAssay-dependent
WBAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control K562 and Jurkat

Properties

Form Liquid
Purification Immunoaffinity chroma-tography
Buffer PBS (pH 7.4) and 0.02% Sodium azide
Preservative 0.02% Sodium azide
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 13368 Mouse DFFB

Swiss-port # O54788 Mouse DNA fragmentation factor subunit beta

Gene Symbol Dffb
Gene Full Name DNA fragmentation factor, beta subunit
Background Cell death signals are transduced by death domain-containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A mouse Dnase that causes DNA fragmentation was identified recently and designated CAD (for caspase activated deoxy-ribonuclease). The human homologue of mouse CAD was more recently identified and termed CPAN, DFF40, and human CAD. DFF45/ICAD is the inhibitory protein of DFF40/CAD with which it forms complexes. Upon cleavage by activated caspase, DFF40/CAD is released and activated and eventually causes the degradation of DNA in the nuclei. Activation of DFF40/CAD is, which causes DNA degradation, is the hallmark of apoptotic cell death.
Function Nuclease that induces DNA fragmentation and chromatin condensation during apoptosis. Degrades naked DNA and induces apoptotic morphology. [UniProt]
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Gene Regulation antibody; Metabolism antibody
Calculated MW 39 kDa

Images (2) Click the Picture to Zoom In

  • ARG54342 anti-DFF40 / CAD antibody ICC/IF image

    Immunofluorescence: Jurkat cells stained with ARG54342 anti-DFF40 / CAD antibody at 5 μg/ml dilution.

  • ARG54342 anti-DFF40 / CAD antibody WB image

    Western blot: H:HeLa; K:K562; J:Jurkat; R:Raji stained with ARG54342 anti-DFF40 / CAD antibody at 1 μg/ml dilution.