ARG10595

anti-EB1 antibody [1A11/4]

anti-EB1 antibody [1A11/4] for IHC-Formalin-fixed paraffin-embedded sections,ICC/IF,Immunoprecipitation,Western blot and Human,Primates,Dog

Overview

Product Description Mouse Monoclonal antibody [1A11/4] recognizes EB1
Tested Reactivity Hu, Dog, NHuPrm
Tested Application ICC/IF, IHC-P, IP, WB
Specificity This antibody recognises a defined epitope which is distinct from the major protein-protein interaction sites. 1A11/4 does not bind EB3 or RP1.
Host Mouse
Clonality Monoclonal
Clone 1A11/4
Isotype IgG1
Target Name EB1
Antigen Species Human
Immunogen Recombinant Human EB1 GST-fusion protein (lack the final 50 aa of EB1)
Conjugation Un-conjugated
Full Name microtubule-associated protein, RP/EB family, member 1
Alternate Names End-binding protein 1; Microtubule-associated protein RP/EB family member 1; EB1; APC-binding protein EB1

Application Instructions

Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS and 0.02% Sodium azide
Preservative 0.02% Sodium azide
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 22919 Human MAPRE1

Swiss-port # Q15691 Human Microtubule-associated protein RP/EB family member 1

Gene Symbol MAPRE1
Gene Full Name microtubule-associated protein, RP/EB family, member 1
Background The protein encoded by this gene was first identified by its binding to the APC protein which is often mutated in familial and sporadic forms of colorectal cancer. This protein localizes to microtubules, especially the growing ends, in interphase cells. During mitosis, the protein is associated with the centrosomes and spindle microtubules. The protein also associates with components of the dynactin complex and the intermediate chain of cytoplasmic dynein. Because of these associations, it is thought that this protein is involved in the regulation of microtubule structures and chromosome stability. This gene is a member of the RP/EB family. [provided by RefSeq, Jul 2008]
Function Binds to the plus end of microtubules and regulates the dynamics of the microtubule cytoskeleton. Promotes cytoplasmic microtubule nucleation and elongation. May be involved in spindle function by stabilizing microtubules and anchoring them at centrosomes. May play a role in cell migration. [UniProt]