ARG10568

anti-ERK1/2 antibody [B20-U]

anti-ERK1/2 antibody [B20-U] for ELISA,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,Immunoprecipitation,Western blot and Human,Mouse,Rat

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anti-ERK1/2 antibody [B20-U]

Rabbit Monoclonal antibody [B20-U] recognizes ERK1/2

ARG10568 Arigo biolaboratories
5.0 out of 5 based on 3
anti-ERK1/2 antibody [B20-U] is rated 5.0 out of 5 by 3.

anti-ERK1/2 antibody [B20-U]

Overview

Product Description

Rabbit Monoclonal antibody [B20-U] recognizes ERK1/2

Tested Reactivity Hu, Ms, Rat
Tested Application ELISA, ICC/IF, IHC-Fr, IHC-P, IP, WB
Host Rabbit
Clonality Monoclonal
Clone B20-U
Isotype IgG
Target Name ERK1/2
Antigen Species Human
Immunogen Synthetic peptide around the C-terminus of Human ERK2.
Conjugation Un-conjugated
Full Name mitogen-activated protein kinase 1
Alternate Names MAPK 2; MAPK 1; MAP kinase 2; ERK-2; p41; ERK; MAP kinase 1; PRKM2; PRKM1; EC 2.7.11.24; MAPK2; p40; Extracellular signal-regulated kinase 2; p38; Mitogen-activated protein kinase 2; Mitogen-activated protein kinase 1; ERK2; MAP kinase isoform p42; p42-MAPK; P42MAPK; p41mapk; ERT1

Application Instructions

Application Suggestion
Tested Application
ELISAAssay-dependent
ICC/IF1:10
IHC-FrAssay-dependent
IHC-PAssay-dependent
IPAssay-dependent
WB1:1000 - 1:5000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Buffer 20 mM Tris-HCl (pH 8.0), 0.05% Sodium azide and 10 mg/ml BSA
Preservative 0.05% Sodium azide
Stabilizer 10 mg/ml BSA
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 116590 Rat MAPK1

GeneID: 26413 Mouse MAPK1

GeneID: 5594 Human MAPK1

Gene Symbol MAPK1
Gene Full Name mitogen-activated protein kinase 1
Background This gene encodes a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. One study also suggests that this protein acts as a transcriptional repressor independent of its kinase activity. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene. [provided by RefSeq, Jan 2014]
Function Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade plays also a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. About 160 substrates have already been discovered for ERKs. Many of these substrates are localized in the nucleus, and seem to participate in the regulation of transcription upon stimulation. However, other substrates are found in the cytosol as well as in other cellular organelles, and those are responsible for processes such as translation, mitosis and apoptosis. Moreover, the MAPK/ERK cascade is also involved in the regulation of the endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC); as well as in the fragmentation of the Golgi apparatus during mitosis. The substrates include transcription factors (such as ATF2, BCL6, ELK1, ERF, FOS, HSF4 or SPZ1), cytoskeletal elements (such as CANX, CTTN, GJA1, MAP2, MAPT, PXN, SORBS3 or STMN1), regulators of apoptosis (such as BAD, BTG2, CASP9, DAPK1, IER3, MCL1 or PPARG), regulators of translation (such as EIF4EBP1) and a variety of other signaling-related molecules (like ARHGEF2, DCC, FRS2 or GRB10). Protein kinases (such as RAF1, RPS6KA1/RSK1, RPS6KA3/RSK2, RPS6KA2/RSK3, RPS6KA6/RSK4, SYK, MKNK1/MNK1, MKNK2/MNK2, RPS6KA5/MSK1, RPS6KA4/MSK2, MAPKAPK3 or MAPKAPK5) and phosphatases (such as DUSP1, DUSP4, DUSP6 or DUSP16) are other substrates which enable the propagation the MAPK/ERK signal to additional cytosolic and nuclear targets, thereby extending the specificity of the cascade. Mediates phosphorylation of TPR in respons to EGF stimulation. May play a role in the spindle assembly checkpoint. Phosphorylates PML and promotes its interaction with PIN1, leading to PML degradation.

Acts as a transcriptional repressor. Binds to a [GC]AAA[GC] consensus sequence. Repress the expression of interferon gamma-induced genes. Seems to bind to the promoter of CCL5, DMP1, IFIH1, IFITM1, IRF7, IRF9, LAMP3, OAS1, OAS2, OAS3 and STAT1. Transcriptional activity is independent of kinase activity. [UniProt]
PTM Phosphorylated upon KIT and FLT3 signaling (By similarity). Dually phosphorylated on Thr-185 and Tyr-187, which activates the enzyme. Undergoes regulatory phosphorylation on additional residues such as Ser-246 and Ser-248 in the kinase insert domain (KID) These phosphorylations, which are probably mediated by more than one kinase, are important for binding of MAPK1/ERK2 to importin-7 (IPO7) and its nuclear translocation. In addition, autophosphorylation of Thr-190 was shown to affect the subcellular localization of MAPK1/ERK2 as well. Ligand-activated ALK induces tyrosine phosphorylation. Dephosphorylated by PTPRJ at Tyr-187. Phosphorylation on Ser-29 by SGK1 results in its activation by enhancing its interaction with MAP2K1/MEK1 and MAP2K2/MEK2. DUSP3 and DUSP6 dephosphorylate specifically MAPK1/ERK2 and MAPK3/ERK1 whereas DUSP9 dephosphorylates a broader range of MAPKs.
ISGylated.

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  • ARG10568 anti-ERK1/2 antibody [B20-U] ICC/IF image

    Immunofluorescence: 100% Methanol fixed (RT, 10 min) HeLa cells stained with ARG10568 anti-ERK1/2 antibody [B20-U] at 1:10 dilution. Left: primary antibody (red). Right: DAPI (blue).

    Secondary antibody: ARG21917 Goat anti-Rabbit IgG antibody (TRITC)

  • ARG10568 anti-ERK1/2 antibody [B20-U] WB image

    Western blot: 20 µg of 293T and Jurkat cell lysates stained with ARG10568 anti-ERK1/2 antibody [B20-U] at 1:5000 dilution.

  • ARG10568 anti-ERK1/2 antibody [B20-U] IP image

    Immunoprecipitation: Erk 1,2 immunoprecipitated from HEK 293 cells, visualized with ARG10568 anti-ERK1/2 antibody [B20-U] in WB analysis at 1:5000 dilution. 1) Marker, 2) 100 µg of HEK 293 stained with ARG10568 anti-ERK1/2 antibody [B20-U] (positive control), 3) Immunoprecipitation without primary antibody (negative control), and 4) Immunoprecipitated Erk 1,2 (~40 and ~45 kDa proteins) from HEK 293 cells (500 µg of crude protein extract) stained with ARG10568 anti-ERK1/2 antibody [B20-U].

  • ARG10568 anti-ERK1/2 antibody [B20-U] WB image

    Western blot: 20 µg of Mouse brain and Rat brain lysates stained with ARG10568 anti-ERK1/2 antibody [B20-U] at 1:5000 dilution.

Customer's Feedback

nuts_pic      Excellent

Review for anti-ERK1/2 antibody [B20-U]

Application:IF/ICC

Sample:HeLa

Fixation Buffer:100% Methanol

Fixation Time:10 min

Fixation Temperature:RT ºC

Permeabilization Buffer:0.1% Triton X-100

Primary Antibody Dilution Factor:1:10

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nuts_pic      Excellent

Review for anti-ERK1/2 antibody [B20-U]

Application:WB

Sample:Mouse brain and Rat brain

Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:1:5000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

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nuts_pic      Excellent

Review for anti-ERK1/2 antibody [B20-U]

Application:WB

Sample:293T and Jurkat

Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:1:5000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

show all review