ARG21499

anti-GM-CSF antibody [MP1-22E9] (azide free)

anti-GM-CSF antibody [MP1-22E9] (azide free) for ELISA,ELISPOT,Flow cytometry,IHC-Frozen sections,ICC/IF,Western blot,Neutralizing and Mouse

Overview

Product Description Azide free and low endotoxin Rat Monoclonal antibody [MP1-22E9] recognizes GM-CSF
Tested Reactivity Ms
Tested Application ELISA, ELISPOT, FACS, ICC/IF, IHC-Fr, Neut, WB
Specificity Mouse GM-CSF.
Host Rat
Clonality Monoclonal
Clone MP1-22E9
Isotype IgG2a, kappa
Target Name GM-CSF
Antigen Species Mouse
Immunogen COS-expressed mouse GM-CSF
Conjugation Un-conjugated
Full Name colony stimulating factor 2 (granulocyte-macrophage)
Alternate Names GM-CSF; Granulocyte-macrophage colony-stimulating factor; CSF; GMCSF; Molgramostin; Colony-stimulating factor; Sargramostim

Application Instructions

Application Suggestion
Tested Application Dilution
ELISA< 5 µg/ml
ELISPOTAssay-dependent
FACSAssay-dependent
ICC/IFAssay-dependent
IHC-FrAssay-dependent
NeutAssay-dependent
WBAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 16 kDa

Properties

Form Liquid
Purification Note Low endotoxin
Buffer PBS
Concentration 0.5 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 12981 Mouse CSF2

Swiss-port # P01587 Mouse Granulocyte-macrophage colony-stimulating factor

Gene Symbol CSF2
Gene Full Name colony stimulating factor 2 (granulocyte-macrophage)
Background The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13. [provided by RefSeq, Jul 2008]
Function Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes. [UniProt]

Images (1) Click the Picture to Zoom In

  • ARG21499 anti-GM-CSF antibody [MP1-22E9] (azide free) standard curve image

    ELISA: Mouse GM-CSF detected by ARG21498 anti-GM-CSF antibody [MP1-22E9] as capture antibody ( ARG21499: Azide free version), and ARG21501 anti-GM-CSF antibody [MP1-31G6] (Biotin) as detection antibody, follow by incubation with streptavidin-HRP.