ARG10169

anti-H. pylori CagA antibody [10E9]

anti-H. pylori CagA antibody [10E9] for ELISA,Western blot and Helicobacter pylori

Microbiology and Infectious Disease antibody

Overview

Product Description

Mouse Monoclonal antibody [10E9] recognizes H. pylori cytotoxin associated gene A protein (CagA)

Tested Reactivity H. pylori
Tested Application ELISA, WB
Specificity Cross-reaction with other proteins has not been identified.
Host Mouse
Clonality Monoclonal
Clone 10E9
Isotype IgG2a,k
Target Name Helicobacter pylori CagA
Immunogen A highly immunogenic 32kD CagA fragment, recombinant
Conjugation Un-conjugated

Application Instructions

Application Note ELISA: Clone 10E9 can be used as capture antibody in sandwich ELISA. Anti-CagA clone 10E9 coated wells detect recombinant CagA antigen in combination with HRP conjugated anti-CagA clone 5C6, clone 3C10 and clone 3C1. In addition, clone 10E9 selectively detected cell lysate of a CagA containing H. pylori strain when HRP conjugated anti-CagA clone 3C10 was used.

Western Blot: This clone detects the 32 kD band corresponding to the molecular weight of the recombinant CagA antigen (Immunogen).

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Protein G affinity purified
Buffer 0.01M PBS (pH 7.0)
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Background Helicobacter pylori (H. pylori), a spiral rod shaped gram-negative bacterium, is frequently found in the stomach. In infected populations, 10-20% may develop gastritis and gastric ulcer and 1-2% may develop cancer. The genome of H. pylori isolates from carriers with symptoms contains a 40kb pathogenicity island encoding H. pylori cytotoxin, cytotoxin associated gene A protein (CagA) and other virulence associated factors. CagA is used as a biomarker for virulent H. pylori strains.
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Resrarch Area Microbiology and Infectious Disease antibody
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