anti-HCV NS3 antibody [24-8]
anti-HCV NS3 antibody [24-8] for ELISA,Western blot and HCV
Microbiology and Infectious Disease antibody
Mouse Monoclonal antibody [24-8] recognizes Hepatitis C Virus NS3
|Tested Application||ELISA, WB|
|Specificity||React with synthetic HCV NS3 protein. (residues 1378-1458 on HCV polyprotein). No cross reaction with HCV polyprotein core region and other non-structural regions.|
|Target Name||Hepatitis C virus NS3|
|Immunogen||A highly antigenic polypeptide consisting of essential sequences of at least 60 residues in length, which were selected from genes encoding the NS-3 region of a Chinese HCV strains|
|Application Note||Western Blot: mAb clone 24-8, when used at a concentration of 4μg/mL, will allow visualization of 200 ng/lane synthetic NS3 protein. The mAb works on lots transferred from both reducing and non-reducing PAGE gel.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
|Purification||Protein G affinity purified|
|Buffer||0.01M PBS (pH 7.2)|
|Storage instruction||For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.|
|Note||For laboratory research only, not for drug, diagnostic or other use.|
|Background||Hepatitis C virus (HCV) causes chronic hepatitis and liver cirrhosis in human through blood and body fluid transmission. HCV has a positive sense single RNA genome enclosed in the nucleocapsid made of core protein (capsid protein). The nucleocapsid is covered by an envelope made of lipoproteins (E1 and E2). The 9.6 kb HCV genome has a single open-reading frame, which is to be translated into a single polyprotein. HCV viral proteins are produced after processing the polyprotein. Genes for core protein and envelop proteins are located adjacently at the 5’-end of HCV genome, followed by genes for non-structural proteins including NS2, NS3, NS4A, NS4B, NS5, NS5A and NS5B.|
|Resrarch Area||Microbiology and Infectious Disease antibody|