anti-HCV NS3 antibody 
anti-HCV NS3 antibody  for ELISA,Western blot and HCV
Microbiology and Infectious Disease antibody
|Product Description||Mouse Monoclonal antibody  recognizes HCV NS3|
|Tested Application||ELISA, WB|
|Specificity||No cross reaction can be seen with recombinant core protein C + envelop protein M (residues 1-142), synthetic core protein C (residues 1-61), synthetic NS-3 protein (residues 1378-1458) and synthetic NS-4a protein (residues 1689-1735).|
|Target Name||HCV NS3|
|Immunogen||Purified recombinant chimeric HCV polyprotein (555 amino acid residues)|
|Application Note||Western Blot: mAb clone 5, when used at 1-10μg/mL will allow visualization of 100 ng/lane of both recombinant chimeric HCV polyprotein and recombinant NS-3 protein. No reaction can be seen with synthetic NS-3. Test was conducted with blots transfer from both reducing and non-reducing PAGE gel.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
|Purification||Protein G affinity purified|
|Buffer||0.01M PBS (pH 7.2)|
|Storage instruction||For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.|
|Note||For laboratory research only, not for drug, diagnostic or other use.|
|Background||Hepatitis C virus (HCV) causes chronic hepatitis and liver cirrhosis in human through blood and body fluid transmission. HCV has a positive sense single RNA genome enclosed in the nucleocapsid made of core protein (capsid protein). The nucleocapsid is covered by an envelope made of lipoproteins (E1 and E2). The 9.6 kb HCV genome has a single open-reading frame, which is to be translated into a single polyprotein. HCV viral proteins are produced after processing the polyprotein. Genes for core protein and envelop proteins are located adjacently at the 5’-end of HCV genome, followed by genes for non-structural proteins including NS2, NS3, NS4A, NS4B, NS5, NS5A and NS5B.|
|Resrarch Area||Microbiology and Infectious Disease antibody|