anti-HCV core antigen antibody [A1/3D1]
anti-HCV core antigen antibody [A1/3D1] for ELISA and HCV
Microbiology and Infectious Disease antibody
Mouse Monoclonal antibody [A1/3D1] recognizes Hepatitis C virus Core Antigen
|Specificity||No cross reaction with recombinant or synthetic HCV non-structural proteins (NS-3 and NS-4).|
|Target Name||Hepatitis C virus core antigen|
|Immunogen||Synthetic peptides derived from HCV core protein|
|Application Note||ELISA: mAb A1/3D1 was tested using indirect ELISA method. The testing plates were coated with HCV non-structural protein (NS), core protein (C) and mixture of NS and C respectively. The neat culture supernatant of hybridoma showed a strong reactivity with HCV capsid protein (OD492 value > 2.0) and with mixture of NS and C (OD492 value >1.5), and showed no reactivity with HCV non-structural protein (OD492 value < 0.03). mAb A1/3D1 recognizes in-vitro translated HCV core protein.
Not suitable for use in Western Blot
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
|Purification||Protein G affinity purified|
|Buffer||0.01M PBS (pH 7.2)|
|Storage instruction||For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.|
|Note||For laboratory research only, not for drug, diagnostic or other use.|
|Background||Hepatitis C virus (HCV) causes chronic hepatitis and liver cirrhosis in human through blood and body fluid transmission. HCV has a positive sense single RNA genome enclosed in the nucleocapsid made of Core Protein (Capsid Protein). The nucleocapsid is covered by an envelope made of lipoproteins (E1 and E2). The 9.6 kb HCV genome has a single open-reading frame, which is to be translated into a single polyprotein. HCV viral proteins are produced after processing the polyprotein. Genes for core protein and envelop proteins are located adjacently at the 5’-end of HCV genome, followed by genes for non-structural proteins including NS2, NS3, NS4A, NS4B, NS5, NS5A and NS5B.|
|Resrarch Area||Microbiology and Infectious Disease antibody|