ARG10097

anti-HLA G antibody [87G]

anti-HLA G antibody [87G] for ELISA,Flow cytometry,Functional study,IHC-Frozen sections and Human

Immune System antibody

Overview

Product Description Mouse Monoclonal antibody [87G] recognizes HLA G
Tested Reactivity Hu
Species Does Not React With Ms, Rat
Tested Application ELISA, FACS, FuncSt, IHC-Fr
Specificity The clone 87G recognizes both membrane-bound and soluble forms of HLA-G (HLA-G1 and HLA-G5). HLA-G belongs to the MHC Class I molecules (MHC Class Ib; nonclassical) and it is expressed on the surface of trophoblast cells.
87G blocks interaction of HLA-G with inhibitory receptors.
Host Mouse
Clonality Monoclonal
Clone 87G
Isotype IgG2a
Target Name HLA G
Antigen Species Human
Immunogen HLA-B27 transgenic mice were imunized with H-2 identical murine cells transfected with and expressing genes encoding HLA-G and human beta2-microglobulin.
Conjugation Un-conjugated
Full Name major histocompatibility complex, class I, G
Alternate Names HLA G antigen; MHC class I antigen G; HLA class I histocompatibility antigen, alpha chain G; MHC-G

Application Instructions

Application Note
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from hybridoma culture supernatant by protein-A affinity chromatography.
Purification Note Fab fragments were prepared by ficin digestion of the IgG followed by protein-A affinity chromatography to remove the remaining intact IgG or Fc fragments.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4), preserved by filter sterilization
Concentration 1 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 3135 Human HLA-G

Swiss-port # P17693 Human HLA class I histocompatibility antigen, alpha chain G

Gene Symbol HLA-G
Gene Full Name major histocompatibility complex, class I, G
Background HLA-G belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-G is expressed on fetal derived placental cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domain, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exon 6 encodes the cytoplasmic tail. [provided by RefSeq, Jul 2008]
Function Involved in the presentation of foreign antigens to the immune system. Plays a role in maternal tolerance of the fetus by mediating protection from the deleterious effects of natural killer cells, cytotoxic T-lymphocytes, macrophages and mononuclear cells. [UniProt]
Research Area Immune System antibody

Images (1) Click the Picture to Zoom In

  • ARG10097 anti-HLA G antibody [87G] Functional study image

    Functional study: Analysis of cytolytical activity of human polyclonal NK cells on target melanoma cells (M8 cell line) transfected with empty vector (Lane 1) or HLA-G1 cDNA (Lane 2-6). Blocking antibodies: Lane (1,2): none Lane (3): Isotype mouse IgG2a control Lane (4): ARG10097 anti-HLA G antibody [87G] (5): ARG10097 anti-HLA G antibody [87G] F(ab´)2 fragment.