anti-HLA G antibody [87G]
anti-HLA G antibody [87G] for ELISA,Flow cytometry,Functional study,IHC-Frozen sections and Human
Immune System antibody
|Product Description||Mouse Monoclonal antibody [87G] recognizes HLA G|
|Species Does Not React With||Ms, Rat|
|Tested Application||ELISA, FACS, FuncSt, IHC-Fr|
|Specificity||The clone 87G recognizes both membrane-bound and soluble forms of HLA-G (HLA-G1 and HLA-G5). HLA-G belongs to the MHC Class I molecules (MHC Class Ib; nonclassical) and it is expressed on the surface of trophoblast cells.
87G blocks interaction of HLA-G with inhibitory receptors.
|Target Name||HLA G|
|Immunogen||HLA-B27 transgenic mice were imunized with H-2 identical murine cells transfected with and expressing genes encoding HLA-G and human beta2-microglobulin.|
|Full Name||major histocompatibility complex, class I, G|
|Alternate Names||HLA G antigen; MHC class I antigen G; HLA class I histocompatibility antigen, alpha chain G; MHC-G|
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
|Purification||Purified from hybridoma culture supernatant by protein-A affinity chromatography.|
|Purification Note||Fab fragments were prepared by ficin digestion of the IgG followed by protein-A affinity chromatography to remove the remaining intact IgG or Fc fragments.|
|Purity||> 95% (by SDS-PAGE)|
|Buffer||PBS (pH 7.4), preserved by filter sterilization|
|Storage instruction||For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.|
|Note||For laboratory research only, not for drug, diagnostic or other use.|
|Gene Full Name||major histocompatibility complex, class I, G|
|Background||HLA-G belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-G is expressed on fetal derived placental cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domain, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exon 6 encodes the cytoplasmic tail. [provided by RefSeq, Jul 2008]|
|Function||Involved in the presentation of foreign antigens to the immune system. Plays a role in maternal tolerance of the fetus by mediating protection from the deleterious effects of natural killer cells, cytotoxic T-lymphocytes, macrophages and mononuclear cells. [UniProt]|
|Research Area||Immune System antibody|
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ARG10097 anti-HLA G antibody [87G] Functional study image
Functional study: Analysis of cytolytical activity of human polyclonal NK cells on target melanoma cells (M8 cell line) transfected with empty vector (Lane 1) or HLA-G1 cDNA (Lane 2-6). Blocking antibodies: Lane (1,2): none Lane (3): Isotype mouse IgG2a control Lane (4): ARG10097 anti-HLA G antibody [87G] (5): ARG10097 anti-HLA G antibody [87G] F(ab´)2 fragment.