ARG10134

anti-Hepatitis B surface antigen / HBs Ag antibody [Hs41]

anti-Hepatitis B surface antigen / HBs Ag antibody [Hs41] for ELISA and Virus

Cancer antibody; Microbiology and Infectious Disease antibody

Overview

Product Description

Mouse Monoclonal antibody [Hs41] recognizes Hepatitis B surface antigen / HBs Ag

Tested Reactivity Virus
Tested Application ELISA
Specificity Reacting with the following HBsAg subtypes: ayw1, ayw2, ayw3, ayw4, ayr, adw2, adw4, adrq+, adrq-, ayw3 (Fer).
Host Mouse
Clonality Monoclonal
Clone Hs41
Isotype IgG1
Target Name Hepatitis B surface antigen / HBs Ag
Immunogen recombinant HBsAg of ayw subtype.
Conjugation Un-conjugated
Full Name L-HBsAG
Alternate Names large S protein; pre-S1/pre-S2/S; L glycoprotein; L-HBsAG; LHB; large surface protein; major surface antigen

Application Instructions

Application Suggestion
Tested Application
ELISAAssay-dependent. (capture - detection): Hs33 - Hs41.
Application Note ELISA sensitivity: 50 pg/ml
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Protein G affinity purified.
Buffer PBS (pH 7.4) and 0.1% Sodium azide
Preservative 0.1% Sodium azide
Concentration 1.0-2.0 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Symbol S
Gene Full Name L-HBsAG
Background Hepatitis B virus (HBV) is a hepadnavirus which has a circular genome composed of partially double-stranded DNA. The HBV surface protein antigens (HBsAg) are comprised of large (LHBs), middle (MHBs) and small (SHBs, also called major) protein. LHBs contains preS1, prS2, and small protein. MHBs does not include preS1 protein and SHBs dose not include preS1 and preS2 proteins. HbsAg and its antibodies have been developed as biomarkers to monitor infection stage. Expression of preS1 and preS2 in tissue or serum are also important to reveal the mechanism of HBV infection.
Function The large envelope protein exists in two topological conformations, one which is termed 'external' or Le-HBsAg and the other 'internal' or Li-HBsAg. In its external conformation the protein attaches the virus to cell receptors and thereby initiating infection. This interaction determines the species specificity and liver tropism. This attachment induces virion internalization predominantly through caveolin-mediated endocytosis. The large envelope protein also assumes fusion between virion membrane and endosomal membrane (Probable). In its internal conformation the protein plays a role in virion morphogenesis and mediates the contact with the nucleocapsid like a matrix protein (By similarity).
The middle envelope protein plays an important role in the budding of the virion. It is involved in the induction of budding in a nucleocapsid independent way. In this process the majority of envelope proteins bud to form subviral lipoprotein particles of 22 nm of diameter that do not contain a nucleocapsid (By similarity). [UniProt]
Highlight Related products:
HBsAg antibodies;
Research Area Cancer antibody; Microbiology and Infectious Disease antibody

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  • ARG10134 anti-Hepatitis B surface antigen / HBs Ag antibody [Hs41] standard curve image

    ARG10134 anti-Hepatitis B surface antigen / HBs Ag antibody [Hs41] results of a typical standard run with optical density reading at 450 nm.

    Capture-detection pair: Hs33 (ARG10828) - Hs41 (ARG10134)