ARG22290

anti-Hsc 70 antibody

anti-Hsc 70 antibody for Western blot,Immunohistochemistry,ICC/IF,Immunoprecipitation,Blocking and Human,Mouse,Rat,Hamster

Overview

Product Description Rabbit Polyclonal antibody recognizes Hsc 70
Tested Reactivity Hu, Ms, Rat, Hm
Tested Application BL, ICC/IF, IHC, IP, WB
Specificity Detects ~73kDa. Does not cross react with HSP70.
Host Rabbit
Clonality Polyclonal
Target Name Hsc 70
Antigen Species Human
Immunogen Synthetic peptide around aa. 650-670 of Human Hsc70
Conjugation Un-conjugated
Alternate Names NIP71; HEL-33; HSPA10; LAP-1; HSC70; HSC71; LAP1; Lipopolysaccharide-associated protein 1; Heat shock 70 kDa protein 8; HSC54; HEL-S-72p; Heat shock cognate 71 kDa protein; HSP71; HSP73; LPS-associated protein 1

Application Instructions

Application Suggestion
Tested Application Dilution
BLAssay-dependent
ICC/IF1:250
IHC1:100
IPAssay-dependent
WB1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Rabbit antiserum.
Buffer Rabbit Antiserum
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 100689472 Hamster HSPA8

GeneID: 15481 Mouse HSPA8

GeneID: 24468 Rat HSPA8

Gene Symbol HSPA8
Gene Full Name heat shock 70kDa protein 8
Background This gene encodes a member of the heat shock protein 70 family, which contains both heat-inducible and constitutively expressed members. This protein belongs to the latter group, which are also referred to as heat-shock cognate proteins. It functions as a chaperone, and binds to nascent polypeptides to facilitate correct folding. It also functions as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]
Function Acts as a repressor of transcriptional activation. Inhibits the transcriptional coactivator activity of CITED1 on Smad-mediated transcription. Chaperone. Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. May have a scaffolding role in the spliceosome assembly as it contacts all other components of the core complex. Binds bacterial lipopolysaccharide (LPS) et mediates LPS-induced inflammatory response, including TNF secretion by monocytes. Participates in the ER-associated degradation (ERAD) quality control pathway in conjunction with J domain-containing co-chaperones and the E3 ligase CHIP. [UniProt]
Cellular Localization Cytoplasm, Melanosome
Calculated MW 71 kDa
PTM Acetylated.
ISGylated.
Trimethylation at Lys-561 reduces fibrillar SNCA binding.

Images (4) Click the Picture to Zoom In

  • ARG22290 anti-Hsc 70 antibody IHC image

    Immunohistochemistry: Formalin fixed Mouse Inflamed colon stained with ARG22290 anti-Hsc 70 antibody at 1:50000 dilution (12 hours). Counterstain: Methyl Green at 200uL for 2 min at RT.

  • ARG22290 anti-Hsc 70 antibody WB image

    Western blot: Human cell line lysates stained with ARG22290 anti-Hsc 70 antibody at 1:1000 dilution.

  • ARG22290 anti-Hsc 70 antibody IHC image

    Immunohistochemistry: Bouin solution fixed Mouse backskin stained with ARG22290 anti-Hsc 70 antibody (green) at 1:100 dilution (1 hour).

  • ARG22290 anti-Hsc 70 antibody IHC image

    Immunohistochemistry: Formalin fixed Human colon carcinoma stained with ARG22290 anti-Hsc 70 antibody at 1:10000 dilution (12 hours). Counterstain: Methyl Green at 200uL for 2 min at RT.