ARG21431

anti-IFN gamma antibody [B27] (azide free)

anti-IFN gamma antibody [B27] (azide free) for ELISA,Flow cytometry,ELISPOT,IHC-Frozen sections,ICC/IF,Immunoprecipitation,Neutralizing,ELISPOT and Human,Rhesus Monkey,Chimpanzee,Macaque,African green monkey

Overview

Product Description Azide free and low endotoxin Mouse Monoclonal antibody [B27 ] recognizes IFN gamma
Tested Reactivity Hu, AGMK, Chimp, Macaq, R. Mk
Tested Application ELISA, ELISPOT, ELISPOT, FACS, ICC/IF, IHC-Fr, IP, Neut
Specificity Human/Rhesus/Cynomolgus/Chimpanzee/Pigtail Macaque/African Green Monkey/Sooty Mangabey IFN-γ.
Host Mouse
Clonality Monoclonal
Clone B27
Isotype IgG1, kappa
Target Name IFN gamma
Antigen Species Human
Immunogen E. coli-expressed human IFN-γ
Conjugation Un-conjugated
Full Name interferon, gamma
Alternate Names IFN-gamma; Interferon gamma; Immune interferon; IFG; IFI

Application Instructions

Application Suggestion
Tested Application Dilution
ELISAAssay-dependent
ELISPOTAssay-dependent
ELISPOTAssay-dependent
FACSAssay-dependent
ICC/IFAssay-dependent
IHC-FrAssay-dependent
IPAssay-dependent
NeutAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 19 kDa

Properties

Form Liquid
Purification Note Low endotoxin
Buffer PBS
Concentration 0.5 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 3458 Human IFNG

Swiss-port # P01579 Human Interferon gamma

Gene Symbol IFNG
Gene Full Name interferon, gamma
Background This gene encodes a soluble cytokine that is a member of the type II interferon class. The encoded protein is secreted by cells of the both the innate and adaptive immune systems. The active protein is a homodimer that binds to the interferon gamma receptor which triggers a cellular response to viral and microbial inflections. Mutations in this gene are associated with an increased susceptibility to viral, bacterial and parasitic infections and to several autoimmune diseases. [provided by RefSeq, Sep 2015]
Function Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. [UniProt]
PTM Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161.