ARG20321

anti-IL1 beta antibody

anti-IL1 beta antibody for Neutralizing,Western blot and Mouse

Pyroptosis Study antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes IL1 beta
Tested Reactivity Ms
Tested Application Neut, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name IL1 beta
Antigen Species Mouse
Immunogen E. coli expressed murine IL1 beta
Conjugation Un-conjugated
Full Name interleukin 1 beta
Alternate Names Interleukin-1 beta; IL1-BETA; IL-1; IL-1 beta; Catabolin; IL1F2

Application Instructions

Application Suggestion
Tested Application Dilution
NeutAssay-dependent
WB0.5-4 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 31 kDa

Properties

Form Liquid
Purification Affinity Purified Antibody
Buffer PBS (pH 7.2), 30% Glycerol, 0.5% BSA and 0.01% Thimerosal
Preservative 0.01% Thimerosal
Stabilizer 30% Glycerol, 0.5% BSA
Concentration 0.5 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 16176 Mouse IL1B

Swiss-port # P10749 Mouse Interleukin-1 beta

Gene Symbol Il1b
Gene Full Name interleukin 1 beta
Background IL1 beta protein is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. [provided by RefSeq, Jul 2008]
Function IL1 beta is a potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells (PubMed:10653850). [UniProt]
Research Area Pyroptosis Study antibody
PTM Activation of the IL1B precursor involves a CASP1-catalyzed proteolytic cleavage. Processing and secretion are temporarily associated.

Images (1) Click the Picture to Zoom In

  • ARG20321 anti-IL-1b antibody WB image

    Western Blot: 1. Recombinant murine IL-1b (10 ng) 2. Recombinant murine IL-1b (50 ng) 3. Recombinant murine IL-1b (250 ng) stained with anti-IL-1 beta antibody (ARG20321).