ARG21477

anti-IL10 antibody [JES5-2A5] (azide free)

anti-IL10 antibody [JES5-2A5] (azide free) for ELISA,ELISPOT,Flow cytometry,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Western blot,Immunoprecipitation,Neutralizing and Mouse

Overview

Product Description Azide free Rat Monoclonal antibody [JES5-2A5 ] recognizes IL10
Tested Reactivity Ms
Tested Application ELISA, ELISPOT, FACS, IHC-Fr, IHC-P, IP, Neut, WB
Specificity Mouse IL-10.
Host Rat
Clonality Monoclonal
Clone JES5-2A5
Isotype IgG1, kappa
Target Name IL10
Antigen Species Mouse
Immunogen E. coli-expressed mouse IL-10
Conjugation Un-conjugated
Full Name interleukin 10
Alternate Names IL10A; GVHDS; TGIF; IL-10; Cytokine synthesis inhibitory factor; CSIF; Interleukin-10

Application Instructions

Application Suggestion
Tested Application Dilution
ELISA< 5 µg/ml
ELISPOTAssay-dependent
FACSAssay-dependent
IHC-FrAssay-dependent
IHC-PAssay-dependent
IPAssay-dependent
NeutAssay-dependent
WBAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 21 kDa

Properties

Form Liquid
Buffer PBS
Concentration 0.5 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 16153 Mouse IL10

Swiss-port # P18893 Mouse Interleukin-10

Gene Symbol IL10
Gene Full Name interleukin 10
Background The protein encoded by this gene is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. Mutations in this gene are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.[provided by RefSeq, May 2011]
Function Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells. [UniProt]