ARG21464

anti-IL4 antibody [BVD4-1D11] (azide free)

anti-IL4 antibody [BVD4-1D11] (azide free) for ELISA,ELISA,ELISPOT,Flow cytometry,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,ICC/IF,Western blot,Immunoprecipitation,Neutralizing and Mouse

Overview

Product Description Azide free and low endotoxin Rat Monoclonal antibody [BVD4-1D11 ] recognizes IL4
Tested Reactivity Ms
Tested Application ELISA, ELISA, ELISPOT, FACS, ICC/IF, IHC-Fr, IHC-P, IP, Neut, WB
Specificity Mouse IL-4.
Host Rat
Clonality Monoclonal
Clone BVD4-1D11
Isotype IgG2b, kappa
Target Name IL4
Antigen Species Mouse
Immunogen E. coli-expressed mouse IL-4
Conjugation Un-conjugated
Full Name interleukin 4
Alternate Names BSF-1; IL-4; B-cell stimulatory factor 1; BCGF1; Lymphocyte stimulatory factor 1; BCGF-1; Interleukin-4; Pitrakinra; Binetrakin; BSF1

Application Instructions

Application Suggestion
Tested Application Dilution
ELISA< 1 µg/ml
ELISA< 1 µg/ml
ELISPOTAssay-dependent
FACSAssay-dependent
ICC/IFAssay-dependent
IHC-FrAssay-dependent
IHC-PAssay-dependent
IPAssay-dependent
NeutAssay-dependent
WBAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 17 kDa

Properties

Form Liquid
Purification Note Low endotoxin
Buffer PBS
Concentration 0.5 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 16189 Mouse IL4

Swiss-port # P07750 Mouse Interleukin-4

Gene Symbol IL4
Gene Full Name interleukin 4
Background The protein encoded by this gene is a pleiotropic cytokine produced by activated T cells. This cytokine is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. This gene, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]
Function Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. [UniProt]

Images (1) Click the Picture to Zoom In

  • ARG21464 anti-IL4 antibody [BVD4-1D11] (azide free) standard curve image

    ELISA: Mouse IL-4 detected by ARG21463 anti-IL4 antibody [BVD4-1D11] as capture antibody ( ARG21464: Azide free version), and ARG21466 anti-IL4 antibody [BVD6-24G2] (Biotin) as detection antibody, follow by incubation with streptavidin-HRP.