ARG20013

anti-Lamin B1 antibody [ZL-5]

anti-Lamin B1 antibody [ZL-5] for ELISA,ICC/IF,Immunoprecipitation,Western blot and Human,Mouse,Rat

Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Controls and Markers antibody; Signaling Transduction antibody; NRF-2 Nuclear Localization Study antibody
nuts_pic
2
anti-Lamin B1 antibody [ZL-5]

Mouse Monoclonal antibody [ZL-5] recognizes Lamin B1

ARG20013 Arigo biolaboratories
4.0 out of 5 based on 2
anti-Lamin B1 antibody [ZL-5] is rated 4.0 out of 5 by 2.

anti-Lamin B1 antibody [ZL-5]

Overview

Product Description

Mouse Monoclonal antibody [ZL-5] recognizes Lamin B1

Tested Reactivity Hu, Ms, Rat
Tested Application ELISA, ICC/IF, IP, WB
Host Mouse
Clonality Monoclonal
Clone ZL-5
Isotype IgG1
Target Name Lamin B1
Species Human
Immunogen Recombinant human Lamin B
Conjugation Un-conjugated
Alternate Names LMN2; ADLD; Lamin-B1; LMN; LMNB

Application Instructions

Application Suggestion
Tested Application Dilution
ELISA0.2-1 µg/ml
ICC/IF10-15 µg/ml
IP5 µg/ml
WB0.5-2 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 66 kDa

Properties

Form Liquid
Purification Affinity Purified Antibody
Buffer PBS, 1 mg/ml BSA, 1.5 mM Sodium azide and 50% Glycerol
Preservative 1.5 mM Sodium azide
Stabilizer 1 mg/ml BSA, 50% Glycerol
Concentration 0.2 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 116685 Rat LMNB1

GeneID: 16906 Mouse LMNB1

GeneID: 4001 Human LMNB1

Background Lamins are classified into A- and B- type isoforms. Specifically, four distinct lamin isoforms have been identified: A, B1, B2, and C. Lamin A and C are alternatively spliced products of a single gene, whereas lamin B1 and B2 are encoded by distinct genes. During mitosis, lamins are phosphorylated by p34cdc2 and solubilized prior to chromosome condensation and nuclear breakdown. A direct role for lamins in chromosome condensation is suggested by the fact that lamin B binds to specific DNA sequence motifs termed matrix attachment regions (MARs). Mars mediate the interaction of chromatin with the nuclear matrix. Importantly, some of the same processes that involve mitotic chromosome condensation and nuclear envelop breakdown are also activated during apoptosis. It appears that chromosome condensation during apoptosis is accompanied by degradation of lamin protein. Lamin degradation occurs prior to DNA fragmentation and this degradation is useful as an apoptotic marker.
Highlight Related Antibody Duos and Panels:
ARG30181 NRF2 Nuclear Localization Antibody Duo (NRF2, Lamin B1)
Related products:
Lamin B1 antibodies; Lamin B1 Duos / Panels; Anti-Mouse IgG secondary antibodies;
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Controls and Markers antibody; Signaling Transduction antibody; NRF-2 Nuclear Localization Study antibody
PTM B-type lamins undergo a series of modifications, such as farnesylation and phosphorylation. Increased phosphorylation of the lamins occurs before envelope disintegration and probably plays a role in regulating lamin associations.

Images (1) Click the Picture to Zoom In

  • ARG20013 anti-Lamin B1 antibody [ZL-5] WB image

    Western blot: 30 µg of PC-3 cell lysate stained with ARG20013 anti-Lamin B1 antibody [ZL-5] at 2 µg/ml dilution.

Customer's Feedback

nuts_pic      Good

Review for anti-Lamin B1 antibody [ZL-5]

Application:WB

Sample:Rat ovary

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:2 µg/ml

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

nuts_pic      Good

Review for anti-Lamin B1 antibody [ZL-5]

Application:WB

Sample:Mouse ovary

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:2 µg/ml

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

Clone References

HO-1 up-regulation: a key point in high-risk neuroblastoma resistance to bortezomib.

WB / 

Furfaro AL et al.
Biochim Biophys Acta.,  (2014)

publication_link

 

hr_line

Tgf-β1 inhibits Cftr biogenesis and prevents functional rescue of ΔF508-Cftr in primary differentiated human bronchial epithelial cells.

WB / Human

Snodgrass SM et al.
PLoS One.,  (2013)

publication_link

 

hr_line

Differential proteomics analysis reveals a role for E2F2 in the regulation of the Ahr pathway in T lymphocytes.

WB / Mouse

Azkargorta M et al.
Mol Cell Proteomics.,  (2010)

publication_link

 

hr_line

Oxidative stress triggers the preferential assembly of base excision repair complexes on open chromatin regions.

WB / Human

Amouroux R et al.
Nucleic Acids Res.,  (2010)

publication_link

 

hr_line

Insulin induces calcium signals in the nucleus of rat hepatocytes.

ICC/IF / Rat

Rodrigues MA et al.
Hepatology.,  (2008)

publication_link

 

hr_line