ARG10719

anti-MAP2 antibody [4H5]

anti-MAP2 antibody [4H5] for ICC/IF,IHC-Frozen sections,Western blot and Human,Mouse,Rat,Bovine

Overview

Product Description Mouse Monoclonal antibody [4H5] recognizes MAP2
Tested Reactivity Hu, Ms, Rat, Bov
Tested Application ICC/IF, IHC-Fr, WB
Host Mouse
Clonality Monoclonal
Clone 4H5
Isotype IgG1
Target Name MAP2
Antigen Species Human
Immunogen Full length purified Bovine protein.
Epitope Epitope mapped to projection domain of Human sequence, between aa. 631 and 1056.
Conjugation Un-conjugated
Full Name microtubule-associated protein 2
Alternate Names MAP2A; Microtubule-associated protein 2; MAP2C; MAP2B; MAP-2

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:1000
IHC-Fr1:1000
WB1:10000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification.
Buffer PBS and 50% Glycerol.
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 17756 Mouse MAP2

GeneID: 25595 Rat MAP2

GeneID: 281294 Bovine MAP2

Gene Symbol MAP2
Gene Full Name microtubule-associated protein 2
Background This gene encodes a protein that belongs to the microtubule-associated protein family. The proteins of this family are thought to be involved in microtubule assembly, which is an essential step in neurogenesis. The products of similar genes in rat and mouse are neuron-specific cytoskeletal proteins that are enriched in dentrites, implicating a role in determining and stabilizing dentritic shape during neuron development. A number of alternatively spliced variants encoding distinct isoforms have been described. [provided by RefSeq, Jan 2010]
Function The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules. [UniProt]
PTM Phosphorylated at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK1 or MARK2), causing detachment from microtubules, and their disassembly (By similarity). Isoform 2 is probably phosphorylated by PKA at Ser-323, Ser-354 and Ser-386 and by FYN at Tyr-67. The interaction with KNDC1 enhances MAP2 threonine phosphorylation (By similarity).

Images (5) Click the Picture to Zoom In

  • ARG10719 anti-MAP2 antibody [4H5] IHC-Fr image

    Immunohistochemistry: Free-floating section of mouse cortex stained with ARG10719 anti-MAP2 antibody [4H5] (green) , and ARG10712 anti-FOX3 / NeuN antibody at 1:2000 dilution. DAPI (blue) used for nuclear staining.

    This antibody labels MAP2 protein in the perikarya and dendrites of most neurons while the FOX3/NeuN antibody selectively stains nuclei and proximal soma of neuronal cells.

  • ARG10719 anti-MAP2 antibody [4H5] ICC/IF image

    Immunocytochemistry: Rat mixed neuron and glia cultures stained with ARG10719 anti-MAP2 antibody [4H5] (green) and co-stained with rabbit antibody to neurofilament NF-H (red); DNA (blue). ARG10719 anti-MAP2 antibody [4H5] reveals strong cytoplasmic staining for of dendrites and perikarya, which does not overlap with the NF-H antibody, which primarily binds to axons.

  • ARG10719 anti-MAP2 antibody [4H5] WB image

    Western blot: Rat brain extract stained with ARG10719 anti-MAP2 antibody [4H5].

  • ARG10719 anti-MAP2 antibody [4H5] WB image

    Western blot: Rat brain, Mouse brain and Rat embryonic cortical neuron-glial cell lysates stained with ARG10719 anti-MAP2 antibody [4H5] (green) at 1:10000 dilution.

  • ARG10719 anti-MAP2 antibody [4H5] WB image

    Western blot: 2 µg of purified recombinant Human MAP2 proteins stained with ARG10719 anti-MAP2 antibody [4H5]. Lanes: [D] MAP2D, [P1] aa. 233-684, [P2] aa. 712-1136, [P3] aa. 1137-1588, [C] MAP2C and [BSA] 2 µg of bovine serum albumin.

    Lanes P1, P2 and P3 show each recombinant segments of the Human MAP2 projection domain, found in MAP2A and B but absent from MAP2C and D.